Document Detail


Immunolocalization of centriole-associated striated rootlets in human submandibular gland cells with and without solitary cilia.
MedLine Citation:
PMID:  12197669     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using an antibody specific to striated rootlets, we investigated the immuolocalization of striated rootlets in cells constituting human submandibular glands. Striated rootlets were positively stained in all cell types constituting acini, intercalated ducts, striated ducts, and interlobular ducts, but their shapes were different. The mean lengths of striated rootlets were 1.46 +/- 0.49, 3.15 +/- 1.35 and 3.99 +/- 1.02 microm in acinar secretory cells, myoepithelial cells, and columnar cells of the striated duct, respectively. The rootlets were the longest in columnar cells of the striated duct, in which paired centrioles were located in the apical cytoplasm away from nuclei. These findings suggest that striated rootlets play important roles in the positioning of centrioles in the cell. 2-8% of striated rootlets in myoepithelial cells were associated with solitary cilia, but they were not associated with solitary cilia in acinar cells and columnar cells of the striated duct. These observations suggest that striated rootlets may be associated with centrioles under normal physiological conditions, without formation of solitary cilia.
Authors:
A Kano; H Hagiwara; K Takata; K Mogi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Histochemical journal     Volume:  33     ISSN:  0018-2214     ISO Abbreviation:  Histochem. J.     Publication Date:    2001 Nov-Dec
Date Detail:
Created Date:  2002-08-28     Completed Date:  2002-09-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0163161     Medline TA:  Histochem J     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  613-20     Citation Subset:  IM    
Affiliation:
Department of Oral and Maxillofacial Surgery, Gunma University School of Medicine, Maebashi, Japan.
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MeSH Terms
Descriptor/Qualifier:
Centrioles / chemistry,  ultrastructure*
Cilia / chemistry,  ultrastructure*
Female
Fluorescent Antibody Technique, Indirect
Humans
Male
Microscopy, Confocal
Microscopy, Immunoelectron
Middle Aged
Submandibular Gland / chemistry,  ultrastructure*
Tubulin / analysis
Chemical
Reg. No./Substance:
0/Tubulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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