| Immunolocalization of the cellular src protein in interphase and mitotic NIH c-src overexpresser cells. | |
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MedLine Citation:
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PMID: 1702788 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mouse mAb, mAb 327, that recognizes specifically both pp60v-src and pp60c-src in a wide variety of cells, has been used to determine precisely the various locations of pp60c-src in NIH c-src overexpresser cells, using the technique of immunofluorescence microscopy. In interphase cells, the protein exhibits two main distributions: one that appears uniform and in association with the cell surface and the other that is patchy and juxtanuclear and coincides with the centrosomes. The juxtanuclear aggregation of pp60c-src-containing patches depends on microtubules and does not seem to occur within the Golgi apparatus and the rough ER. At the G2-to-M-phase transition, a drastic change in the localization patterns of pp60c-src takes place. We also report experiments in which the NIH c-src overexpresser cells were exposed to Con A for various times to induce a redistribution of the cell surface Con A receptors. We show that, at each stage of the Con A-mediated endocytotic process, the Con A-receptor complexes redistribute into structures to which pp60c-src appears also to be associated: at first, into patches that form at the cell surface level and then, into a cap that stands at the cell center in a juxtanuclear position and that coincides with the Golgi apparatus. During this capping process, pp60c-src-containing vesicles continue to accumulate in a centriolar spot, as in interphase, Con A-untreated cells, from which Con A is excluded. The significance of the intracellular locations of pp60c-src to the possible functions of the protein is discussed. Also, the distribution patterns of the cellular protein in the NIH c-src overexpresser cells are compared with those of pp60v-src in RSV-transformed cells. The differences observed are discussed in relation with the differences in transforming capacities of the two proteins. Finally, the possible physiological significance of the association between pp60c-src and the structures generated after the binding of Con A to its surface receptors is addressed. |
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Authors:
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T David-Pfeuty; Y Nouvian-Dooghe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of cell biology Volume: 111 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1990 Dec |
Date Detail:
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Created Date: 1991-02-21 Completed Date: 1991-02-21 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3097-116 Citation Subset: IM |
Affiliation:
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Institut Curie-Biologie, Centre Universitaire, Orsay, France. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Adhesion / physiology Cell Compartmentation Cell Line Concanavalin A / metabolism Endocytosis / physiology Fluorescent Dyes Immunohistochemistry Interphase / physiology* Mitosis / physiology* Proto-Oncogene Proteins pp60(c-src) / analysis*, physiology Recombinant Proteins / analysis Transfection |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Recombinant Proteins; 11028-71-0/Concanavalin A; EC 2.7.10.2/Proto-Oncogene Proteins pp60(c-src) |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 12 Year: 1990 Volume: 111 Issue: 6 First Page: 3097 Last Page: 3116 ID: 2116410 Publisher Id: 91100422 PubMed Id: 1702788 |
| Immunolocalization of the cellular src protein in interphase and mitotic NIH c-src overexpresser cells | |
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