Document Detail

Immunolocalization of calcitriol receptor, 24-hydroxylase cytochrome P-450, and calbindin D28k in human kidney.
MedLine Citation:
PMID:  8160797     Owner:  NLM     Status:  MEDLINE    
The precise localization of the calcitriol (1 alpha,25-dihydroxyvitamin D3) receptor (VDR) and the 25-hydroxyvitamin D3 [25(OH)D3] 24-hydroxylase cytochrome P-450 in the human kidney is unknown. Using newly developed polyclonal antibodies against the human VDR, we demonstrate that the receptor is present in cells of the distal tubule, the collecting duct, the proximal tubule, and in the parietal epithelial cells of the glomerulus. In the distal tubule and collecting duct not all cells contain epitopes for the receptor. The protein is not detected in glomerular capillaries, in the glomerular mesangium, in the interstitium, or in blood vessels. Specific polyclonal antibodies directed against the 25(OH)D3 24-hydroxylase cytochrome P-450 demonstrate epitopes for the cytochrome in cells of the proximal tubule, the distal tubule, glomerular parietal epithelial cells, and mesangial cells. The protein is absent from interstitial cells. Calbindin D28k is present exclusively in principal cells of the distal tubule and collecting duct. In the human kidney, the VDR is present in cells where vitamin D-inducible proteins are found; conversely it is absent from cells where vitamin D-dependent proteins are not present.
R Kumar; J Schaefer; J P Grande; P C Roche
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  266     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1994 Mar 
Date Detail:
Created Date:  1994-05-19     Completed Date:  1994-05-19     Revised Date:  2014-01-13    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  F477-85     Citation Subset:  IM    
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MeSH Terms
Base Sequence
Calbindin 1
Cytochrome P-450 Enzyme System / genetics,  metabolism*
Kidney / metabolism*
Molecular Sequence Data
Molecular Weight
Oligonucleotide Probes / genetics
Receptors, Calcitriol / metabolism*
S100 Calcium Binding Protein G / chemistry,  metabolism*
Steroid Hydroxylases / genetics,  metabolism*
Grant Support
Reg. No./Substance:
0/CALB1 protein, human; 0/Calb1 protein, rat; 0/Calbindin 1; 0/Calbindins; 0/Oligonucleotide Probes; 0/Receptors, Calcitriol; 0/S100 Calcium Binding Protein G; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.14.-/Steroid Hydroxylases; EC D 24-hydroxylase

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