| Immunolocalization of androgen receptor and estrogen receptors alpha and beta in human fetal testis and epididymis. | |
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MedLine Citation:
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PMID: 16148684 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: Expression and cellular localization of the androgen receptor (AR) and estrogen receptor (ER) isoforms were determined using antibodies specific to these receptors and to specific cell types. MATERIALS AND METHODS: Gonads and genitourinary structures were removed from 5 human male fetuses 7 to 22 weeks of gestational age. Sections were stained with antibodies to AR, ERalpha and ERbeta, P450 scc and smooth muscle actin. RESULTS: AR was present in undifferentiated gonadal cells, peritubular myoid cells and in some Leydig and stromal cells at 7 weeks of gestation. The number of AR positive peritubular myoid cells remained constant through 22 weeks of gestation but the number of AR positive stromal cells continued to increase through 22 weeks. ERalpha was apparent by 12 weeks of gestation with perinuclear staining of Leydig cells, peaked at 16 weeks and then diminished. ERbeta was first observed at 7 weeks in undifferentiated gonadal cells. By 12 weeks of gestation ERbeta was apparent in germ cells, PTMC and Leydig cells. In the epididymis AR was expressed in the epithelium and stroma of the efferent ductules and the ductus epididymis by 7 weeks of gestation with increased expression by 12 weeks. A similar pattern of staining was observed for ERbeta. By contrast, staining of ERalpha was observed only in the epithelium of the epididymis from 7 weeks of gestation onward with no apparent ERalpha staining in the tail of the epididymis. CONCLUSIONS: These findings are compatible with the well-known roles of androgen signaling in sexual differentiation and spermatogenesis in humans. The role of estrogens in the developing human testis and epididymis remains unknown. |
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Authors:
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Ellen Shapiro; Hongying Huang; Rachel J Masch; Deborah E McFadden; Xue-Ru Wu; Harry Ostrer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of urology Volume: 174 ISSN: 0022-5347 ISO Abbreviation: J. Urol. Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-09-08 Completed Date: 2005-10-27 Revised Date: 2006-09-18 |
Medline Journal Info:
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Nlm Unique ID: 0376374 Medline TA: J Urol Country: United States |
Other Details:
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Languages: eng Pagination: 1695-8; discussion 1698 Citation Subset: AIM; IM |
Affiliation:
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Department of Urology, New York University School of Medicine, New York, New York 10016, USA. ellen.shapiro@med.nyu.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Epididymis
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immunology* Estrogen Receptor alpha / immunology* Estrogen Receptor beta / immunology* Fetus Gestational Age Humans Immunoenzyme Techniques Male Receptors, Androgen / immunology* Testis / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Estrogen Receptor alpha; 0/Estrogen Receptor beta; 0/Receptors, Androgen |
| Comments/Corrections | |
Erratum In:
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J Urol. 2005 Dec;174(6):2428 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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