Document Detail


Immunolocalization of 3beta-HSD and 17beta-HSD in the testis of the spotted ray Torpedo marmorata.
MedLine Citation:
PMID:  17561019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using polyclonal antibodies, we examined the localization of 3beta-hydroxysteroid dehydrogenase (3beta-HSD) and 17beta-hydroxysteroid dehydrogenase (17beta-HSD) as markers of the site of steroidogenetic activity during the spermatogenesis of Torpedo marmorata. These enzymes play a central role in the biosynthesis of steroid hormones, including androgen and oestrogen production. We demonstrated that in the spotted ray testis, Sertoli and Leydig cells, as well as spermatogonia, show a positive reaction to anti 3beta-HSD and 17beta-HSD antibodies. In particular, we demonstrated that Sertoli cells show a positive reaction to anti 3beta-HSD and 17beta-HSD antibodies in cysts containing spermatogonia and spermatozoa, while Leydig cells present a positive reaction only when they are located between cysts containing meiotic cells. This study strongly suggests that, as hypothesised in our previous study [Prisco, M., Liguoro, A., D'Onghia, B., Ricchiari, L., Andreuccetti, P., Angelini, F., 2002. Fine structure of Leydig and Sertoli cells in the testis of immature and mature spotted ray Torpedo marmorata. Mol. Reprod. Dev. 63, 192-201.], Sertoli and Leydig cells are differently involved in the hormonal control of spermatogenesis: Sertoli cells before the beginning of meiosis and after spermiation, Leydig cells only during meiosis phase. Moreover, the present paper deals with the possibility that also spermatogonia are engaged in the production of androgen hormones, as they are characterized by the presence of 3beta-HSD and 17beta-HSD enzymes, and show the ultrastructural features of steroid hormone-producing cells.
Authors:
Marina Prisco; Annamaria Liguoro; Loredana Ricchiari; Giuseppina Del Giudice; Francesco Angelini; Piero Andreuccetti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-01
Journal Detail:
Title:  General and comparative endocrinology     Volume:  155     ISSN:  0016-6480     ISO Abbreviation:  Gen. Comp. Endocrinol.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-11-26     Completed Date:  2008-02-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370735     Medline TA:  Gen Comp Endocrinol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  157-63     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Evolutionary and Comparative Biology Division, University of Naples Federico II, Via Mezzocannone 8, Naples 80134, Italy.
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MeSH Terms
Descriptor/Qualifier:
17-Hydroxysteroid Dehydrogenases / metabolism*
3-Hydroxysteroid Dehydrogenases / metabolism*
Animals
Immunohistochemistry
Male
Meiosis / physiology
Spermatogenesis / physiology
Spermatogonia / metabolism
Testis / metabolism*
Torpedo / metabolism*
Chemical
Reg. No./Substance:
EC 1.1.-/17-Hydroxysteroid Dehydrogenases; EC 1.1.-/3-Hydroxysteroid Dehydrogenases; EC 1.1.1.51/3 (or 17)-beta-hydroxysteroid dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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