| Immunohistological and biochemical evidence for a role for hyaluronic acid in the growth and development of the placenta. | |
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MedLine Citation:
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PMID: 3912503 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A monoclonal antibody, designated NDOG1, has been used to stain a series of human and monkey placentae as well as several adult human tissues using immunoperoxidase techniques. In early placentae, NDOG1 was found to stain extracellular material associated with proliferating, extravillous cytotrophoblast cell columns and with the cytotrophoblast shell at the feto-maternal junction. The immunohistology suggests that NDOG1 antigen may be secreted by the anchoring cytotrophoblast into the immediately adjacent maternal tissues. NDOG1 antibody also shows extracellular staining in the stroma of early human placentae and reacted with the apical villous syncytiotrophoblast plasma membrane throughout pregnancy. Biochemical experiments demonstrated that extracts of this latter membrane contained NDOG1 antigenic activity which was susceptible to digestion with bovine testicular hyaluronidase. Hyaluronic acid was the only glycosaminoglycan found in this membrane, thereby implying a reaction between NDOG1 antibody and hyaluronic acid. Whilst no such direct interaction could be demonstrated in vitro, NDOG1 was shown to compete with two other antibodies which themselves demonstrated specificity for hyaluronic acid. The proposed identity between the NDOG1 antigen and hyaluronic acid is discussed particularly in terms of placentation where the distribution of NDOG1 staining may confirm the role of hyaluronic acid in providing an open matrix structure during stages of cell proliferation, migration and invasion. |
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Authors:
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C A Sunderland; J N Bulmer; M Luscombe; C W Redman; G M Stirrat |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of reproductive immunology Volume: 8 ISSN: 0165-0378 ISO Abbreviation: J. Reprod. Immunol. Publication Date: 1985 Nov |
Date Detail:
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Created Date: 1986-04-02 Completed Date: 1986-04-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8001906 Medline TA: J Reprod Immunol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 197-212 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Animals Antibodies, Monoclonal Female Fetus / physiology Gestational Age Glycosaminoglycans / analysis Humans Hyaluronic Acid / physiology* Immunoenzyme Techniques Macaca fascicularis Placenta / cytology, physiology* Pregnancy Species Specificity Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Glycosaminoglycans; 9004-61-9/Hyaluronic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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