Document Detail


Immunohistochemistry of chromogranin A and B, and secretogranin II in the canine endocrine pancreas.
MedLine Citation:
PMID:  2505828     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The chromogranins are large acidic proteins contained in secretion granules of adrenal medullary cells. These proteins are also presumed to be regular constituents of other endocrine cells, e.g., pancreatic endocrine cells. In our previous immunohistochemical studies performed in the endocrine pancreas of 10 mammalian species, only canine endocrine cells lacked any immunoreactivity for chromogranin A. Therefore, this problem was reinvestigated in the present study. Antisera against bovine and rat chromogranin A, B, and C (= secretogranin II) were applied under various conditions of the immunohistochemical protocol. Upon meeting certain requirements, chromogranin-immunoreactivities were found also in canine pancreatic B- (insulin), PP- (pancreatic polypeptide), and EC- (enterochromaffin) cells. The immunohistochemical data suggest that canine chromogranins partially differ structurally or immunologically from bovine chromogranins and completely from rat chromogranins. In addition, the present findings confirm our previous findings about both interspecies differences in the cellular localization of chromogranins in the endocrine pancreas and the peculiar localization of secretogranin II to pancreatic PP-cells. Finally, the present methodological studies have shown that even the buffer used in the immunohistochemical protocol may be decisive for a false-positive or false-negative immunostaining.
Authors:
D Grube; S Yoshie
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives of histology and cytology     Volume:  52     ISSN:  0914-9465     ISO Abbreviation:  Arch. Histol. Cytol.     Publication Date:  1989 Jul 
Date Detail:
Created Date:  1989-10-20     Completed Date:  1989-10-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8806082     Medline TA:  Arch Histol Cytol     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  287-98     Citation Subset:  IM    
Affiliation:
Department of Anatomy, Hanover Medical School, Federal Republic of Germany.
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MeSH Terms
Descriptor/Qualifier:
Adrenal Medulla / analysis
Animals
Cattle
Chromogranin A
Chromogranins / analysis*
Cross Reactions
Dogs
Enterochromaffin Cells / analysis
Immune Sera
Immunohistochemistry
Islets of Langerhans / analysis*
Male
Microscopy, Fluorescence
Nerve Tissue Proteins / analysis*
Pancreatic Polypeptide / analysis
Proteins / analysis*
Rats
Serotonin / analysis,  immunology
Chemical
Reg. No./Substance:
0/Chromogranin A; 0/Chromogranins; 0/Immune Sera; 0/Nerve Tissue Proteins; 0/Proteins; 0/chromogranin A, rat; 50-67-9/Serotonin; 59763-91-6/Pancreatic Polypeptide

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