Document Detail

Immunohistochemical and ultrastructural investigation of neural differentiation in Ewing sarcoma/PNET of bone and soft tissues.
MedLine Citation:
PMID:  11465478     Owner:  NLM     Status:  MEDLINE    
The authors evaluated the role of immunohistochemistry and electron microscopy in defining neural differentiation in 28 cases of Ewing sarcoma/PNET. The panel of primary antibodies used included vimentin, MIC-2, NSE, S-100 protein, leu7, neurofilaments, GFAP, and chromogranin A. Cases were considered undifferentiated when neural markers were absent, poorly differentiated if one neural marker was present, and well differentiated if two or more markers were observed. Cases were also evaluated for the presence of cytoplasmic processes, microtubules, and neurosecretory granules as ultrastructural features of neural differentiation: the tumor was classified as well differentiated if two of these features were present; and poorly differentiated if one was evident; all other cases were considered undifferentiated. According to immunohistochemistry, 10 cases (35.7%) were undifferentiated, 12 cases (42.9%) were poorly differentiated, and 6 (21.4%) were well differentiated. According to the ultrastructural analysis, 10 tumors were undifferentiated (35.7%), 14 poorly differentiated (50%), and 4 well differentiated (14.3%). The overall concordance between the two techniques was low (35.7%), and both modalities were concordant in classifying only 1 well-differentiated, 5 poorly differentiated, and 4 undifferentiated tumors. In conclusion, the authors suggest that investigations devoted to test the prognostic significance of neural differentiation in these neoplasms should employ both immunohistochemistry and electron microscopy, separately and in combination, to assess what is the most effective choice for predicting the clinical course.
A Franchi; G Pasquinelli; G Cenacchi; C Della Rocca; C Gambini; M Bisceglia; G N Martinelli; M Santucci
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Ultrastructural pathology     Volume:  25     ISSN:  0191-3123     ISO Abbreviation:  Ultrastruct Pathol     Publication Date:    2001 May-Jun
Date Detail:
Created Date:  2001-07-23     Completed Date:  2001-12-07     Revised Date:  2009-06-26    
Medline Journal Info:
Nlm Unique ID:  8002867     Medline TA:  Ultrastruct Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  219-25     Citation Subset:  IM    
Dipartimento di Patologia Umana ed Oncologia, Università degli Studi de Firenze, Italy.
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MeSH Terms
Bone Neoplasms / metabolism,  ultrastructure*
Cell Differentiation
Child, Preschool
Microscopy, Electron*
Middle Aged
Nerve Tissue / metabolism,  ultrastructure*
Neuroectodermal Tumors, Primitive / metabolism,  ultrastructure*
Sarcoma, Ewing's / metabolism,  ultrastructure*
Soft Tissue Neoplasms / metabolism,  ultrastructure*

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