Document Detail

Immunohistochemical study on the localization of the epitope defined by a human saliva-specific mouse monoclonal antibody (P4-5C).
MedLine Citation:
PMID:  1716968     Owner:  NLM     Status:  MEDLINE    
A novel mouse monoclonal antibody (P4-5C) has been developed which recognizes the core portion of the protein carrying ABO(H) blood group antigens in human saliva. This proved to be specific for human saliva using immunochemical investigations such as enzyme-linked immunosorbent assay, Ouchterlony method and counter-immunoelectrophoresis. By light and electron microscope studies with immunohistochemical techniques using this human saliva-specific P4-5C as primary antibody, it was shown that P4-5C reacted specifically and exclusively with mucus from the mucous gland cells of human salivary glands. P4-5C reacted neither with the mucous gland cells of other primates (hamadryas baboon, Japanese monkey and Rhesus monkey) and four mammals (dog, cat, rabbit and mouse) nor with other human tissues. The epitope on the core portion of the ABO(H)-carrying protein was defined by P4-5C and could be discriminated from the epitope of ABO(H) blood group antigens using immunoelectronmicroscopy, although these 2 epitopes were localized relatively close to each other. The P4-5C monoclonal antibody can be also used for morphological species identification of tissue specimens from submandibular glands.
T Ohshima; T Nagano; A Kimura; F Matsumura; K Sodesaki; T Tsuji; H Maeda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of legal medicine     Volume:  104     ISSN:  0937-9827     ISO Abbreviation:  Int. J. Legal Med.     Publication Date:  1991  
Date Detail:
Created Date:  1991-11-08     Completed Date:  1991-11-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9101456     Medline TA:  Int J Legal Med     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  137-40     Citation Subset:  IM    
Department of Legal Medicine, Kanazawa University School of Medicine, Japan.
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MeSH Terms
ABO Blood-Group System / immunology
Antibodies, Monoclonal / diagnostic use*
Epitopes / analysis*
Saliva / chemistry*
Species Specificity
Submandibular Gland / chemistry*,  ultrastructure
Reg. No./Substance:
0/ABO Blood-Group System; 0/Antibodies, Monoclonal; 0/Epitopes

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