| Immunohistochemical study of the distribution of sodium-dependent vitamin C transporters in adult rat brain. | |
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MedLine Citation:
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PMID: 16477646 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Sodium-dependent vitamin C transporters (SVCTs) is known to transport the reduced form of ascorbic acid into the cell, whereas the oxidized form of vitamin C (VC) is moved through a facilitative sugar transporter, such as glucose transporter (GLUT). With regard to the distribution of SVCT1 and -2 within the various organs, they were reported to be expressed in different types of cells. Especially in the central nervous system, only SVCT2 mRNA was expressed mainly in neurons and some types of neuroglial cells. However, data on the expression of SVCT proteins in the brain are scant. Therefore, we tried to develop comprehensive data on the distribution of SVCT proteins in adult rat brain by using immunohistochemical techniques for the first time. In our study, SVCT2 immunoreactivities (IRs) were intensely localized in the neurons of cerebral cortex, hippocampus, and Purkinje cells of cerebellum, and much weaker SVCT2 IRs were found in the other brain regions. Judging from double-immunohistochemical data, most of the cells expressing SVCT2 IRs were likely to be neurons or microglia, even though the cells in choroids plexus or ependymal cells around the ventricles also exhibited SVCT2 IRs. Complete mapping of the distribution of SVCT2 IRs was available by using a semiquantitative method. The subcellular localization of SVCT proteins is necessary for understanding the exact role of the protein, so the current overall mapping of SVCT IRs in the rat brain could be the basis for further studies on related subjects. |
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Authors:
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Ga Hee Mun; Myeung Ju Kim; Ju Hyun Lee; Hyun Jun Kim; Yoon Hee Chung; Young Bae Chung; Jae Seung Kang; Young Il Hwang; Seung Ha Oh; Joong-Gon Kim; Douk Ho Hwang; Dong Hoon Shin; Wang Jae Lee |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of neuroscience research Volume: 83 ISSN: 0360-4012 ISO Abbreviation: J. Neurosci. Res. Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-03-23 Completed Date: 2006-06-01 Revised Date: 2011-01-21 |
Medline Journal Info:
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Nlm Unique ID: 7600111 Medline TA: J Neurosci Res Country: United States |
Other Details:
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Languages: eng Pagination: 919-28 Citation Subset: IM |
Affiliation:
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Department of Anatomy, Seoul National University College of Medicine, Seoul, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Brain / metabolism* Immunohistochemistry Microglia / metabolism Neurons / metabolism Organic Anion Transporters, Sodium-Dependent / metabolism* Rats Reverse Transcriptase Polymerase Chain Reaction Symporters / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Organic Anion Transporters, Sodium-Dependent; 0/Symporters; 0/sodium-dependent vitamin C transporter |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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