Document Detail

Immunohistochemical study of desmosomes in acne vulgaris.
MedLine Citation:
PMID:  8011498     Owner:  NLM     Status:  MEDLINE    
Desmosomes contribute towards adhesion between adjacent keratinocytes. In acne vulgaris, increased intercellular adhesion is thought to contribute to the retention of keratinocytes within the follicular lumen during comedogenesis. Therefore, the distribution of different desmosomal components was investigated in normal and acne subjects. Biopsies were cryostat-sectioned (6 microns), and stained with antibodies to different desmosomal components: desmoplakin 1/2, desmoglein 1, desmocollin 3a/3b, and a late desmosomal antigen, G36-19. Desmoplakin 1/2, desmoglein 1 and desmocollin 3a/3b shared a similar distribution in follicles from control skin, from acne-affected skin, and in non-inflamed lesions. All three proteins were expressed around the periphery of keratinocytes of all the intrafollicular epidermis, except the basal lamina and the upper stratum corneum. In inflamed lesions, the expression of desmoglein 1 and desmocollin 3a/3b was diminished; in 12.5%, staining for these two proteins was completely abolished, and in 81.25% of the lesions investigated the staining was patchy. The antibody G36-19 bound to an antigen in the upper granular layer in the infundibular epidermis. No differences were noted in the staining pattern of the follicular epithelia of controls, non-inflamed, and inflamed lesions. This study, using monoclonal antibodies, did not identify any changes in the desmosomal components which might explain the increased adhesion between follicular keratinocytes during comedogenesis.
H E Knaggs; B R Hughes; C Morris; E J Wood; D B Holland; W J Cunliffe
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The British journal of dermatology     Volume:  130     ISSN:  0007-0963     ISO Abbreviation:  Br. J. Dermatol.     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-07-28     Completed Date:  1994-07-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0004041     Medline TA:  Br J Dermatol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  731-7     Citation Subset:  IM    
Department of Dermatology, General Infirmary at Leeds, U.K.
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MeSH Terms
Acne Vulgaris / immunology,  metabolism*,  pathology
Antibodies, Monoclonal
Antigens / analysis
Cytoskeletal Proteins / analysis
Desmoglein 1
Desmosomes / chemistry*,  immunology,  pathology
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens; 0/Cytoskeletal Proteins; 0/DSG1 protein, human; 0/DSP protein, human; 0/Desmocollins; 0/Desmoglein 1; 0/Desmogleins; 0/Desmoplakins

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