Document Detail


Immunohistochemical study on the delayed progression of epithelial apoptosis in follicle-associated epithelium of rat Peyer's patch.
MedLine Citation:
PMID:  18057826     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It is well known that some caspases in apoptosis is involved in determinant of terminal differentiation and maturation of various cells. Our previous study ultrastructurally clarified the differentiation into M cells from immature microvillous epithelial cells and the redifferentiation from M cells to microvillous epithelial cells in the follicle-associated epithelium (FAE) of rat Peyer's patch. In this study, the difference of epithelial apoptosis between the FAE of Peyer's patch and intestinal villi was immunohistochemically investigated in rat jejunoileum. As a result, cleaved caspase-3 was limited to several epithelial cells at the tip of FAE, whereas almost all of the epithelial cells were cleaved caspase-3 positive in intestinal villi. Cleaved caspase-9 was detected only in a few exfoliating or exfoliated epithelial cells of both FAE and intestinal villi. Nuclear DNA-fragmentation was detected only in several epithelial cells of the tip of FAE, while it was expressed from the middle regions in the intestinal villi. The DNase I expression of the epithelial cytoplasm was much weaker in FAE than in intestinal villi. Bcl-x expression was restricted in the apical cytoplasms of epithelial cells in the FAE, whereas it was restricted in whole cytoplasms in villous epithelial cells. These findings suggest that the progression of the apoptotic process in the epithelial cells of FAE is later than in the intestinal villi, so that the possibility of epithelial differentiation might be remained in the FAE, unlike in the intestinal villi.
Authors:
Sachiko Onishi; Hidenori Miyata; Tetsurou Inamoto; Wang-Mei Qi; Kenkichi Yamamoto; Toshifumi Yokoyama; Katsuhiko Warita; Nobuhiko Hoshi; Hiroshi Kitagawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of veterinary medical science / the Japanese Society of Veterinary Science     Volume:  69     ISSN:  0916-7250     ISO Abbreviation:  J. Vet. Med. Sci.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-12-06     Completed Date:  2008-01-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9105360     Medline TA:  J Vet Med Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1123-9     Citation Subset:  IM    
Affiliation:
Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University, kobe 657-8501, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / physiology*
Caspase 3 / genetics,  metabolism
Caspase 9 / genetics,  metabolism
DNA Fragmentation
Deoxyribonuclease I / genetics,  metabolism
Epithelial Cells / cytology*,  physiology
Gene Expression Regulation
Immunohistochemistry*
Intestinal Mucosa / cytology*,  physiology
Male
Peyer's Patches / cytology*,  physiology
Rats
Rats, Wistar
bcl-X Protein / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Bcl2l1 protein, rat; 0/bcl-X Protein; EC 3.1.21.1/Deoxyribonuclease I; EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Casp9 protein, rat; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9

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