| Immunohistochemical study of NGF and its receptors in the synovial membrane of the ankle joint of adjuvant-induced arthritic rats. | |
| | |
MedLine Citation:
|
PMID: 11201606 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
To study the role of nerve growth factor (NGF) in local inflammation, we investigated the expression of NGF and its receptors, trkA and p75, in the ankle joints of adjuvant-induced arthritic rats. Infiltrated mononuclear cells revealed a positive immunoreactivity for NGF and trkA; they were also positive for immunostaining for W3/25 and ED1, which mainly stain T cells and macrophages, respectively. Changes in the ratios of NGF-positive cells to mononuclear cells showed a relatively similar pattern for trkA-positive cells, which peaked at weeks 2 to 3 after the adjuvant injection. In double-immunofluorescence staining, 80% and 65% of NGF-positive cells stained for W3/25 and ED1, respectively. Similarly, 67% and 80% of trkA-positive cells also corresponded to W3/25- and ED1-positive cells, respectively. However, p75 immunoreactivity localized on the nerve fibers but not on the cells of the ankle joints. Dense meshworks of p75-positive nerve fibers with numerous terminal varicosities were observed at weeks 2 to 4. The present findings suggest that infiltrated mononuclear cells may secrete NGF in an autocrine or paracrine manner in the inflamed synovium. An upregulation of NGF in these mononuclear cells and an increase in density of the synovial nerve fibers may be involved in the development of adjuvant arthritis in rats. |
| | |
Authors:
|
Z Wu; K Nagata; T Iijima |
Related Documents
:
|
8466596 - Pc12 cells release stimulatory factors after transfection with beta/a4-c-terminal dna o... 15325966 - Alpha and beta estradiol protect neuronal but not native pc12 cells from paraquat-induc... 20578246 - Neurite outgrowth from pc12 cells by basic fibroblast growth factor (bfgf) is mediated ... 20157396 - Glycyrrhizin attenuates mptp neurotoxicity in mouse and mpp-induced cell death in pc12 ... 18045786 - Endonet: an information resource about regulatory networks of cell-to-cell communication. 12237396 - Two distinct sources of elicited reactive oxygen species in tobacco epidermal cells. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Histochemistry and cell biology Volume: 114 ISSN: 0948-6143 ISO Abbreviation: Histochem. Cell Biol. Publication Date: 2000 Dec |
Date Detail:
|
Created Date: 2001-01-30 Completed Date: 2001-03-15 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 9506663 Medline TA: Histochem Cell Biol Country: Germany |
Other Details:
|
Languages: eng Pagination: 453-9 Citation Subset: IM |
Affiliation:
|
Department of Oral Anatomy and Cell Biology, Faculty of Dental Science, Kyushu University, Fukuoka, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Ankle Joint / chemistry, innervation, pathology Arthritis, Experimental / immunology, pathology* Immunohistochemistry Leukocytes, Mononuclear / immunology Nerve Fibers / chemistry, immunology, pathology Nerve Growth Factor / analysis*, immunology Paracrine Communication / immunology Rats Receptor, Nerve Growth Factor / analysis*, immunology Receptor, trkA / analysis*, immunology Synovial Membrane / chemistry*, innervation, pathology Synovitis / immunology, pathology |
| Chemical | |
Reg. No./Substance:
|
0/Receptor, Nerve Growth Factor; 9061-61-4/Nerve Growth Factor; EC 2.7.10.1/Receptor, trkA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Dual enhancement of double immunofluorescent signals by CARD: participation of ubiquitin during form...
Next Document: Immunohistochemical evaluation of endomannosidase distribution in rat tissues: evidence for cell typ...