Document Detail

Immunohistochemical studies on the expression pattern of molecular chaperones HSC70 and HSP25 and cell cycle-related proteins cyclin D1 and PCNA in rat liver after thioacetamide intoxication.
MedLine Citation:
PMID:  12376827     Owner:  NLM     Status:  MEDLINE    
Intoxication of rats with thioacetamide (TAA) is a model system to investigate mechanisms involved in liver cell death and tissue reconstitution. Our study was undertaken to determine by immunohistochemistry the expression pattern of the cytoprotective chaperone proteins HSC70 and HSP25 and proliferation markers cyclin D1 and PCNA in livers of Wistar rats intraperitoneally injected with TAA at a single dose of 50 mg/kg. For each protein studied we observed distinct dynamic changes in appearance and localization in liver lobules. During 24-36 h after TAA injection the HSC70 cytoplasmic immunoreaction gradually disappeared from hepatocytes localized around central veins and a shift of immunostaining to cell nuclei took place. Then, 36-48 h after TAA injection the HSC70 cytoplasmic immunoreaction reappeared with the highest intensity in hepatocytes surrounding the areas of inflammatory cells. HSP25, undetectable in control hepatocytes began to appear at approximately 36 h after TAA injection and HSP25-immunopositive cells formed a characteristic ring around areas of inflammation. Of the proteins studied, the most rapid reaction to TAA was observed for cyclin D1. As early as 15 min after TAA administration cyclin D1-positive hepatocytes appeared in intermediate and periportal areas of liver lobules and a subsequent shift of staining to centrilobular hepatocytes took place at 36 and 48 h. There was no correlation of cyclin D1 localization either with PCNA-positive cells or mitotic cells. Our observations suggest that in TAA-treated livers HSP25 and HSC70 proteins can play an anti-inflammatory role, and the early and distinct cyclin D1 expression is not related to proliferation of hepatocytes.
Anna Zborek; Ewa Małusecka; Stefania Krzyowska-Gruca; Aleksandra Wysocka; Zdzisław Krawczyk
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2002-09-05
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  118     ISSN:  0948-6143     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-10-11     Completed Date:  2003-06-03     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  311-9     Citation Subset:  IM    
Department of Tumor Biology, Centre of Oncology, Maria Skłodowska-Curie Memorial Institute, Wybrzee Armii Krajowej 15, 44-100 Gliwice, Poland.
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MeSH Terms
Blotting, Northern
Blotting, Western
Cyclin D1 / metabolism*
Disease Models, Animal
Drug-Induced Liver Injury / metabolism*,  pathology
HSC70 Heat-Shock Proteins
HSP27 Heat-Shock Proteins
HSP70 Heat-Shock Proteins / metabolism*
Heat-Shock Proteins*
Immunoenzyme Techniques
Injections, Intraperitoneal
Liver / metabolism*,  pathology
Neoplasm Proteins / metabolism*
Proliferating Cell Nuclear Antigen / metabolism*
Rats, Wistar
Thioacetamide / administration & dosage,  toxicity
Time Factors
Reg. No./Substance:
0/HSC70 Heat-Shock Proteins; 0/HSP27 Heat-Shock Proteins; 0/HSP70 Heat-Shock Proteins; 0/Heat-Shock Proteins; 0/Hspa8 protein, rat; 0/Hspb1 protein, rat; 0/Neoplasm Proteins; 0/Proliferating Cell Nuclear Antigen; 136601-57-5/Cyclin D1; 62-55-5/Thioacetamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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