| Immunohistochemical studies on the distribution of cellular myosin II isoforms in brain and aorta. | |
MedLine Citation:
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PMID: 1387589 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The distribution of nonmuscle myosin isoforms in brain and aorta was studied by using polyclonal antibodies against two synthetic peptides selected from a region near the carboxyl terminus of bovine brain (peptide IIB) and human macrophage (peptide IIA) myosin. Immunoblots of brain homogenates and purified myosin showed two major bands stained by anti-peptide IIB (MIIB1 and MIIB2) and a minor band stained by anti-peptide IIA (MIIA2). Polyclonal anti-human platelet myosin antibodies did not react with MIIB isoforms. In cryosections from bovine, rat, and mouse brains, anti-peptide IIB stained most neuronal cells. In bovine cryosections, glial staining was also observed. In contrast, anti-peptide IIA and anti-platelet myosin antibodies primarily stained blood vessels. In bovine aorta, the anti-peptide antibodies recognized four bands, MIIB3, MIIB4, MIIA1, and MIIA2. Only MIIA2 was recognized by anti-human platelet myosin antibodies. In bovine aorta cryosections, anti-peptide IIB stained smooth muscle cells in tunica intima and tunica media but did not stain endothelial cells. Anti-peptide IIA stained smooth muscle cells in the tunica media, and endothelial cells of vaso vasorum but not of aorta. Only polyclonal anti-platelet myosin antibodies stained the endothelial cells of aorta tunica intima. These results indicate that multiple isoforms of cellular myosins exist in mammals, that these isoforms are expressed in a cell specific manner, and that the major myosin isoforms isolated from whole brain originate from neurons and, at least in bovine brain, from glia, but not from blood vessels. |
Authors:
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N Murakami; M Elzinga |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cell motility and the cytoskeleton Volume: 22 ISSN: 0886-1544 ISO Abbreviation: Cell Motil. Cytoskeleton Publication Date: 1992 |
Date Detail:
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Created Date: 1992-10-06 Completed Date: 1992-10-06 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8605339 Medline TA: Cell Motil Cytoskeleton Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 281-95 Citation Subset: IM |
Affiliation:
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Laboratory of Neurobiochemistry, New York State Institute for Basic Research in Developmental Disabilities, Staten Island 10314. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Aorta / cytology, enzymology* Brain / cytology Brain Chemistry / physiology* Cattle Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Immunoblotting Immunoenzyme Techniques Mice Molecular Sequence Data Myosins / analysis* Rats Sequence Homology, Nucleic Acid |
| Grant Support | |
ID/Acronym/Agency:
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HL-21471/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 3.6.4.1/Myosins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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