Document Detail

Immunohistochemical staining patterns of keratins in normal oesophageal epithelium and carcinoma of the oesophagus.
MedLine Citation:
PMID:  7536179     Owner:  NLM     Status:  MEDLINE    
To clarify the keratin staining patterns of invasive carcinoma of the oesophagus, 22 cases of formalin-fixed paraffin-embedded surgical specimens were examined immunohistochemically with the labelled streptavidin biotin method using a panel of six different monoclonal anti-keratin antibodies. The antibody reacted adequately when antigen was retrieved in a microwave oven, and the relationship between morphological characteristics and keratin reaction patterns was analyzed in carcinomas and compared with adjacent histologically normal epithelium. In the normal oesophageal epithelium, AE3 and CK8.12 labelled all layer of cells, KS-1A3, E3 and KL1 labelled suprabasal cells, and LL002 selectively labelled the basal cells. In squamous cell carcinomas, AE3, CK8.12, KL1 and LL002 labelled almost all the tumour cells regardless of their differentiation, E3 only labelled keratinized cells, while marked decrease or loss of KS-1A3 staining was seen in all cases examined. Therefore, the characteristic profile of squamous cell carcinoma was a strong and diffuse expression of keratin 14 and 16, strong but localized expression of keratin 17, and loss of keratin 13 expression. Undifferentiated carcinoma totally lacked all keratin reactivity. The findings suggested that the neoplastic epithelial cells showed different keratin reactivity and distribution compared to normal oesophageal epithelium. In addition, histologically normal epithelium, dysplasia and carcinoma-in-situ adjacent to or overlying carcinoma expressed keratin 14.
H Takahashi; N Shikata; H Senzaki; M Shintaku; A Tsubura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Histopathology     Volume:  26     ISSN:  0309-0167     ISO Abbreviation:  Histopathology     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-05-17     Completed Date:  1995-05-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7704136     Medline TA:  Histopathology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  45-50     Citation Subset:  IM    
Department of Pathology Kansai Medical University, Osaka, Japan.
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MeSH Terms
Antibodies, Monoclonal / immunology
Carcinoma / chemistry*
Carcinoma, Squamous Cell / chemistry
Esophageal Neoplasms / chemistry*
Esophagus / chemistry*
Immunoenzyme Techniques
Keratins / analysis*
Reg. No./Substance:
0/Antibodies, Monoclonal; 68238-35-7/Keratins

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