Document Detail


Immunohistochemical and morphometric analysis of calcitonin distribution in anuran ultimobranchial glands.
MedLine Citation:
PMID:  3264539     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The presence and distribution of immunoreactive calcitonin (iCT) in the anuran (Rana pipiens) ultimobranchial gland was examined with a specific antiserum to salmon calcitonin. Improved resolution of the distribution of iCT within individual cells allowed recognition of a iCT-negative [iCT(-)] early maturing cell, the primary iCT-positive [iCT-(+)] secretory cell with accumulation of iCT within the basal and apical cytoplasm, an iCT(-) cell that lacks a basal accumulation of secretory material, and a iCT(-) cell that borders the central lumen. A morphometric analysis was made of the entire glandular basal secretory surface area and a calculation was made of the relative area that contained iCT(+) secretory material (expressed as a basal index (BI)). For frogs kept in fresh water, the total basal area of a single gland was 0.335 mm2, with a BI of 0.72 and a total iCT(+) secretory surface of 0.241 mm2. A second group of frogs kept in a high calcium medium for 12 days had a BI of 0.74 with no significant increase in parenchyma volume or total basal area and therefore no increase in the total iCT(+) secretory area. A third group was kept in a high calcium medium for 12 days and given a single oral dose of vitamin D3 (500 IU/g body wt). The BI of this group remained unchanged at 0.75; however, there was a significant glandular hypertrophy concomitant with an increase in secretory surface area to 0.462 mm2. This resulted in a 43% increase in the iCT(+) secretory area to 0.345 mm2. A model is proposed to explain the increase in iCT in the gland in response to a vitamin D and high calcium challenge.
Authors:
D R Robertson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  General and comparative endocrinology     Volume:  71     ISSN:  0016-6480     ISO Abbreviation:  Gen. Comp. Endocrinol.     Publication Date:  1988 Aug 
Date Detail:
Created Date:  1989-02-09     Completed Date:  1989-02-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370735     Medline TA:  Gen Comp Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  349-58     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Cell Biology, State University of New York Health Science Center, Syracuse 13210.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcitonin / analysis*
Calcium / pharmacology
Hyperplasia
Immunohistochemistry
Male
Rana pipiens / anatomy & histology,  metabolism*
Ultimobranchial Body / analysis*,  cytology
Chemical
Reg. No./Substance:
7440-70-2/Calcium; 9007-12-9/Calcitonin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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