Document Detail

Immunohistochemical manifestations of unilateral kidney ischemia.
MedLine Citation:
PMID:  8996759     Owner:  NLM     Status:  MEDLINE    
Events in the early post-transplant period have been correlated with increased renal allograft loss. Immunologic reactions and ischemic injury have been implicated in this process. While the immunologic aspects of allograft injury have been studied extensively, ischemic effects remain less well understood. To study the effects of ischemia in rats with different genetic backgrounds without the introduction of an alloimmune response, a clamp was placed on the vascular pedicle of the left kidney for 60 min. The short-term effects (1 wk) of ischemia were studied in groups of PVG (RT1c), LEW (RT1), DA (RT1a) and WR (RT1u) rats, Immunoperoxidase staining demonstrated limited infiltration of monocytes, macrophages, and T-cells accompanied by upregulation of low levels of MHC class II antigens on tubular epithelial cells, peritubular capillaries, and interstitial cells in kidneys of PVG and WF rats. Kidneys of LEW and DA rats had greater influxes of monocytes, macrophages, and T cells in addition to higher amounts of MHC class II antigens upregulation on tubular epithelium and interstitial cells. The long-term effects of ischemia were studied in kidneys of WF rats. These kidneys had a progressive increase in infiltrating T cells, monocytes, macrophages and MHC class II expression on the tubular epithelium and the interstitial cells at 14, 30, and 90 d after the ischemic insult. The differences in MHC class II expression between ischemic kidneys of PVG and LEW rats were not associated with differences in production of mRNA for IL-2, IFN-gamma, and TNF-alpha. In summary, transient renal ischemia in the absence of an allogeneic immune response triggers a progression of inflammatory responses, including leukocyte infiltration, cytokine production and MHC class II antigen upregulation which appears to be strain-dependent.
S Ibrahim; F Jacobs; Y Zukin; D Enriquez; D Holt; W Baldwin; F Sanfilippo; L E Ratner
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Clinical transplantation     Volume:  10     ISSN:  0902-0063     ISO Abbreviation:  Clin Transplant     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-03-20     Completed Date:  1997-03-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8710240     Medline TA:  Clin Transplant     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  646-52     Citation Subset:  IM    
Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21205, USA.
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MeSH Terms
Disease Models, Animal
Graft Survival
Immunoenzyme Techniques
Kidney / blood supply*
Kidney Transplantation / adverse effects*
Rats, Inbred Lew / genetics
Rats, Inbred Strains / genetics*
Rats, Inbred WF / genetics
Rats, Sprague-Dawley / genetics
Rats, Wistar / genetics
Reperfusion Injury / immunology*,  pathology*
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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