Document Detail

Immunohistochemical localization of several cytoskeletal proteins in inner ear sensory and supporting cells.
MedLine Citation:
PMID:  6178719     Owner:  NLM     Status:  MEDLINE    
Several structural and contractile proteins have been searched for with immunohistochemical methods using antibodies directed against these proteins. Three types of preparations from the guinea pig have been used: isolated stereocilia from the utricle, organ of Corti fragments obtained by cellular dissociation and 0.2-1 micrometer sections obtained by cryoultramicrotomy. The main finding is that different sets of proteins compose the cytoskeleton in supporting cells and the mechanoreceptor structures of the sensory cells. Thus, actin was found in association with fimbrin in the mechanoreceptive region of hair cells, whereas supporting cells, although rich in actin, did not reveal fimbrin. Instead tubulin was seen together with actin in supporting cells which also exhibited prekeratin. Fimbrin appears to function as a protein capable of making bundles and networks from actin filaments. Its exclusive presence in the mechanosensitive region of the sensory cells is possibly related to the function of these cells as mechanoreceptors.
A Flock; A Bretscher; K Weber
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hearing research     Volume:  7     ISSN:  0378-5955     ISO Abbreviation:  Hear. Res.     Publication Date:  1982 May 
Date Detail:
Created Date:  1982-09-24     Completed Date:  1982-09-24     Revised Date:  2008-09-03    
Medline Journal Info:
Nlm Unique ID:  7900445     Medline TA:  Hear Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  75-89     Citation Subset:  IM; S    
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MeSH Terms
Actins / analysis
Fluorescent Antibody Technique
Frozen Sections
Guinea Pigs
Hair Cells, Auditory / analysis*
Keratins / analysis
Labyrinth Supporting Cells / analysis*
Membrane Glycoproteins*
Membrane Proteins / analysis
Microfilament Proteins*
Organ of Corti / analysis*
Protein Precursors / analysis
Proteins / analysis*
Saccule and Utricle / analysis
Tubulin / analysis
Reg. No./Substance:
0/Actins; 0/Membrane Glycoproteins; 0/Membrane Proteins; 0/Microfilament Proteins; 0/Protein Precursors; 0/Proteins; 0/Tubulin; 0/plastin; 0/prekeratin; 68238-35-7/Keratins

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