Document Detail


Immunohistochemical localization of pyruvate carboxylase and carbamyl-phosphate synthetase I in normal and neoplastic human pancreatic tissues.
MedLine Citation:
PMID:  12142734     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: It has been suggested that pyruvate carboxylase (PC) and carbamyl-phosphate synthetase I (CPS I) might be colocalized with carbonic anhydrase V (CA V), which is generally considered to provide HCO3- ions for PC and CPS I. AIM: To examine the immunohistochemical staining of endogenous biotin; of three mitochondrial biotin-binding enzymes (namely, PC, CPS I, and propionyl CoA-carboxylase); and of cytosolic acetyl CoA-carboxylase in pancreatic tissues. METHODOLOGY: Immunohistochemical analysis was performed on 23 samples of normal pancreas and 63 samples of neoplastic pancreatic tissues. RESULTS: It was found that the distribution of PC, CPS I, and endogenous biotin was not related to that of CA V but was similar to that of CA II in normal centroacinar cells, intercalated duct cells, and intralobular duct cells. In addition, PC was detected unexpectedly in delta-cells of islets. CONCLUSION: It seems likely that CA II plays a major role in the secretion of NaHCO3 into the pancreatic juice. Hence, it is possible that PC and CPS I in the centroacinar cells, intercalated duct cells, and intralobular duct cells are strongly activated and might use HCO3- ions provided by CA II and not by CA V. Among the pancreatic neoplasms examined, ductal adenocarcinomas exhibited significantly elevated immunoreactivity specific for the four biotin-binding enzymes.
Authors:
Tetsuro Sato; Kenji Kashima; Ayako Gamachi; Tsutomu Daa; Iwao Nakayama; Shigeo Yokoyama
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pancreas     Volume:  25     ISSN:  1536-4828     ISO Abbreviation:  Pancreas     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-07-26     Completed Date:  2002-08-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8608542     Medline TA:  Pancreas     Country:  United States    
Other Details:
Languages:  eng     Pagination:  130-5     Citation Subset:  IM    
Affiliation:
Department of Pathology, Oita Medical University, Oita, Japan. satot@oita-med.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Acetyl-CoA Carboxylase / metabolism
Blotting, Western
Carbamoyl-Phosphate Synthase (Ammonia) / metabolism*
Carboxy-Lyases / metabolism
Humans
Immunohistochemistry
Methylmalonyl-CoA Decarboxylase
Microscopy, Immunoelectron
Pancreas / enzymology*,  ultrastructure
Pancreatic Neoplasms / enzymology*,  pathology,  ultrastructure
Pyruvate Carboxylase / metabolism*
Chemical
Reg. No./Substance:
EC 4.1.1.-/Carboxy-Lyases; EC 4.1.1.41/Methylmalonyl-CoA Decarboxylase; EC 6.3.4.16/Carbamoyl-Phosphate Synthase (Ammonia); EC 6.4.1.1/Pyruvate Carboxylase; EC 6.4.1.2/Acetyl-CoA Carboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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