Document Detail


Immunohistochemical localization of prostaglandin synthase in the rat uterus and embryo during the peri-implantation period.
MedLine Citation:
PMID:  3129038     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Prostaglandins (PGs) appear to have a role in the appearance of the increased uterine vascular permeability and subsequent decidualization observed at implantation in many species. However, the sites of production of these PGs have not been clearly established. To clarify the PG synthetic capacity of the blastocyst and the various types of cells in the uterus at implantation, we have studied the immunohistochemical localization of PG synthase in the rat blastocyst on Days 5 to 7 and uterus on Days 1, 4, 5, 6, and 7 of pregnancy. Labeling of PG synthase was negligible in the uterus on Day 1 of pregnancy. On Day 4, there was increased labeling in the luminal and glandular epithelium, in stromal cells adjacent to the luminal epithelium, and in blood vessels and some leukocytes. PG synthase was detected in the blastocysts on Days 5 to 7, but there was a gradual loss of label in the luminal and glandular epithelial cells during this period. Early differentiating stromal cells adjacent to the luminal epithelium in the implantation site on Day 5 showed bright labeling, whereas peripheral stromal cells were only slightly labeled. By Day 7, the differentiated cells of the primary decidual zone showed little or no label, but cells in the secondary decidual zone were brightly labeled. These results indicate that PG synthase is present in the rat blastocyst and in several kinds of uterine cells, and that its localization in uterine cells changed markedly during the implantation process.
Authors:
M B Parr; E L Parr; K Munaretto; M R Clark; S K Dey
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biology of reproduction     Volume:  38     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1988 Mar 
Date Detail:
Created Date:  1988-06-08     Completed Date:  1988-06-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  333-43     Citation Subset:  IM    
Affiliation:
Department of Anatomy, Southern Illinois University School of Medicine, Carbondale 62901.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastocyst / enzymology*
Embryo Implantation*
Endothelium, Vascular / enzymology
Epithelium / enzymology
Female
Fluorescent Antibody Technique
Immunohistochemistry
Pregnancy
Prostaglandin-Endoperoxide Synthases / analysis*
Rats
Time Factors
Tissue Distribution
Uterus / blood supply,  enzymology*
Grant Support
ID/Acronym/Agency:
HD-13073/HD/NICHD NIH HHS; HD-17480/HD/NICHD NIH HHS; HD-22299/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases

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