Document Detail

Immunohistochemical localization of mitochondrial fatty acid β-oxidation enzymes in Müller cells of the retina.
MedLine Citation:
PMID:  21046137     Owner:  NLM     Status:  MEDLINE    
The presence of a mitochondrial fatty acid β-oxidation system in the retina was shown by immunohistochemistry. Fatty acids are considered to serve as a major energy source metabolized by fatty acid β-oxidation together with glucose metabolized by glycolysis in the organs of the entire body, but almost nothing is known about this metabolic system in the retina. Adult rat retinae were subjected to immunofluorescence and immuno-electron microscopy for the localization of fatty acid β-oxidation enzymes, together with western blot analysis for quantitation of the amount of enzyme proteins and DNA microarray analysis for gene expression. All the enzymes examined were shown to be present in the retina, but in small amounts, with the amount of protein and gene expression in the retina being about 1/10 of those in the liver. Immunohistochemistry at light and electron microscopic levels revealed the enzymes to be more preferentially localized to the mitochondria of Müller cells than the retinal neurons. The Müller cells were isolated from the retina and confirmed for the presence of mitochondrial fatty acid β-oxidation enzymes. A mitochondrial fatty acid β-oxidation system was thus shown to be present in the retina heterogeneously.
Kimie Atsuzawa; Ayami Nakazawa; Kenmei Mizutani; Motoaki Fukasawa; Naoki Yamamoto; Takashi Hashimoto; Nobuteru Usuda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-11-03
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  134     ISSN:  1432-119X     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-29     Completed Date:  2011-03-24     Revised Date:  2012-11-16    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  565-79     Citation Subset:  IM    
Department of Anatomy II and Cell Biology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
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MeSH Terms
Fatty Acids / metabolism*
Microscopy, Immunoelectron
Mitochondria / enzymology*
Multienzyme Complexes / metabolism*
Neuroglia / enzymology*
Rats, Wistar
Retina / enzymology*
Reg. No./Substance:
0/Fatty Acids; 0/Multienzyme Complexes; 0/mitochondrial fatty acid beta-oxidation trifunctional protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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