Document Detail

Immunohistochemical localization of metallothionein in dental pulp after cavity preparation of rat molars.
MedLine Citation:
PMID:  17656138     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Metallothionein (MT) is an intracellular cysteine-rich protein associated with cell proliferation and differentiation. Our objective was to examine immunohistochemically the localization of MT in the rat dental pulp after cavity preparation. STUDY DESIGN: Cavities were prepared on the upper first molars of 9 rats. Specimens were collected at 1, 3, and 5 days after cavity preparation, and paraffin sections were made. For double-immunohistochemical staining, anti-MT monoclonal antibody (E9) and anti-proliferating cell nuclear antigen (PCNA) monoclonal antibody (PC10) were applied. RESULTS: At 3 days after cavity preparation, some odontoblasts corresponding to the cavity, many pulp cells, and some endothelial cells in the pulp under the cavity showed both MT- and PCNA-positive immunostainings. CONCLUSION: Metallothionein was detected in the dental pulp after pulp injury, and it is likely that MT is closely related to the proliferation of newly differentiating odontoblasts and angiogenesis during the healing process.
Toshio Izumi; Tarou Eida; Noriyoshi Matsumoto; Hiroshi Inoue
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-07-26
Journal Detail:
Title:  Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics     Volume:  104     ISSN:  1528-395X     ISO Abbreviation:  Oral Surg Oral Med Oral Pathol Oral Radiol Endod     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-01     Completed Date:  2007-10-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508562     Medline TA:  Oral Surg Oral Med Oral Pathol Oral Radiol Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e133-7     Citation Subset:  D; IM    
Department of Operative Dentistry and Endodontology, Fukuoka Dental College, Fukuoka, Japan.
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MeSH Terms
Cell Differentiation
Cell Proliferation
Dental Cavity Preparation*
Dental Pulp / chemistry*
Dentin, Secondary / secretion
Immunoenzyme Techniques
Metallothionein / analysis*
Neovascularization, Physiologic
Odontoblasts / chemistry
Proliferating Cell Nuclear Antigen / analysis
Rats, Sprague-Dawley
Wound Healing / physiology*
Reg. No./Substance:
0/Proliferating Cell Nuclear Antigen; 9038-94-2/Metallothionein

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