Document Detail

Immunohistochemical localization of growth factors fibroblast growth factor-1 and fibroblast growth factor-2 and receptors fibroblast growth factor receptor-2 and fibroblast growth factor receptor-3 in normal oral epithelium, epithelial dysplasias, and squamous cell carcinoma.
MedLine Citation:
PMID:  12075207     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: Fibroblast growth factors (FGFs) and their receptors (FGFRs) have been identified in a variety of carcinomas, but there are few studies concerning their presence in oral cancers. The objective of this study was to determine whether FGF-1, FGF-2, and high affinity receptors FGFR2 and FGFR3 are present in the pathogenesis of oral epithelial dysplasias and oral squamous cell carcinoma.
STUDY DESIGN: Sections from formalin-fixed, paraffin-embedded samples of oral normal mucosa (n = 14), epithelial dysplasia (n = 20), carcinoma in situ (n = 10), and squamous cell carcinoma (n = 12) were tested for cytoplasmic staining by standard in situ immunohistochemistry with antibodies for FGF-1, FGF-2, FGFR2, and FGFR3.
RESULTS: Staining for FGF-1 is decreased or lost in the development of epithelial dysplasia and carcinoma. Staining for FGF-2 showed increased intensity (although not statistically significant) in oral epithelial dysplasias and squamous cell carcinomas and showed a significant increased expression in the upper layers of dysplasias and stratum spinosum-like cells in squamous cell carcinomas. Staining for FGFR2 showed a statistically significant increase in intensity in all layers of epithelial dysplasias and squamous cell carcinomas. Staining for FGFR3 was found in the upper stratum spinosum cells of normal and dysplastic epithelium and well-differentiated squamous cells in squamous cell carcinomas, with a statistically significant increase in staining intensity in dysplastic and carcinomatous tissues.
CONCLUSIONS: The loss of FGF-1 is consistent with loss of differentiation in dysplasias and some squamous cell carcinomas. Changes in the localization of FGF-2 and FGFR2 into upper epithelial layers with increasing dysplasia suggest increased mitotic potential of high level cells. The co-localization of FGF-2 and its high affinity receptors in neoplastic tissues suggests an autocrine mechanism of influence on carcinogenesis.
Candice Wakulich; Linda Jackson-Boeters; Tom D Daley; George P Wysocki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics     Volume:  93     ISSN:  1079-2104     ISO Abbreviation:  Oral Surg Oral Med Oral Pathol Oral Radiol Endod     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-06-20     Completed Date:  2002-09-03     Revised Date:  2012-06-05    
Medline Journal Info:
Nlm Unique ID:  9508562     Medline TA:  Oral Surg Oral Med Oral Pathol Oral Radiol Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  573-9     Citation Subset:  D; IM    
University of Western Ontario, London, Ontario, Canada.
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MeSH Terms
Antibodies / diagnostic use
Autocrine Communication
Biological Markers / analysis
Carcinoma in Situ / pathology
Carcinoma, Squamous Cell / pathology*
Cell Differentiation
Coloring Agents / diagnostic use
Cytoplasm / ultrastructure
Epithelium / pathology
Fibroblast Growth Factor 1 / analysis*
Fibroblast Growth Factor 2 / analysis*
Matched-Pair Analysis
Mouth Mucosa / cytology*
Mouth Neoplasms / pathology*
Protein-Tyrosine Kinases*
Receptor Protein-Tyrosine Kinases / analysis*
Receptor, Fibroblast Growth Factor, Type 2
Receptor, Fibroblast Growth Factor, Type 3
Receptors, Fibroblast Growth Factor / analysis*
Statistics, Nonparametric
Tumor Markers, Biological / analysis
Reg. No./Substance:
0/Antibodies; 0/Biological Markers; 0/Coloring Agents; 0/Receptors, Fibroblast Growth Factor; 0/Tumor Markers, Biological; 103107-01-3/Fibroblast Growth Factor 2; 104781-85-3/Fibroblast Growth Factor 1; EC protein, human; EC protein, human; EC Kinases; EC Protein-Tyrosine Kinases; EC, Fibroblast Growth Factor, Type 2; EC, Fibroblast Growth Factor, Type 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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