| Immunohistochemical localization of galectins-1 and -3 and monitoring of tissue galectin-binding sites during tubular regeneration after renal ischemia reperfusion in the rat. | |
| | |
MedLine Citation:
|
PMID: 20865664 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Endogenous lectins act as effectors of cellular activities such as growth regulation, migration and adhesion. In this study, we report the histochemical detection of galectins and their binding sites in rat kidneys after ischemic injury (35 min) with regard to renal regeneration. In this context, we have shown in a previous publication (Vansthertem et al., 2008) that extrarenal cells (CD44+, vimentin +) could be involved in this process of tubular restoration. In controls, galectin-1 is expressed by fusiform-shaped cells within cortical and medullar interstitium. Two days after ischemia, the number of positive interstitial cells increased temporarily within OSOM in the vicinity of altered tubules to later reach control level. After ischemia, we identified a population of galectin-3 (+), CD44 (+), and vimentin (+) interstitial round cells located in the outer stripe of outer medulla (OSOM) in the vicinity of necrotic tubules, but also in the lumen of adjacent blood vessels. The immunocytochemical characteristics of theses cells, along with their distribution within OSOM, suggest the involvement of a unique cell population during kidney regeneration. On the other hand, the distribution and density of binding sites for galectins within OSOM were not modified after ischemia and remained similar to controls. Altogether, our observations suggest that galectin-3 may be involved in the complex process of kidney regeneration following ischemia/reperfusion injury. |
| | |
Authors:
|
David Vansthertem; Stéphanie Cludts; Denis Nonclercq; Annabel Gossiaux; Sven Saussez; Alexandre Legrand; Hans-Joachim Gabius; Gérard Toubeau |
Related Documents
:
|
8548484 - Effect of glomerular cells interaction with macrophages on surface 5'-nucleotidase acti... 12937224 - Glomerular epithelial-mesenchymal transdifferentiation in pauci-immune crescentic glome... 7853754 - Contribution of ed-1- and cd-8-positive cells to the development of crescentic-type ant... 22276214 - Spire, an actin nucleation factor, regulates cell division during drosophila heart deve... 10726974 - Expression of apoptosis and cell cycle related genes in proliferating and colcemid arre... 8447934 - The mechanisms of action of cyclosporin a in the treatment of psoriasis. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Histology and histopathology Volume: 25 ISSN: 1699-5848 ISO Abbreviation: Histol. Histopathol. Publication Date: 2010 Nov |
Date Detail:
|
Created Date: 2010-09-24 Completed Date: 2011-01-03 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8609357 Medline TA: Histol Histopathol Country: Spain |
Other Details:
|
Languages: eng Pagination: 1417-29 Citation Subset: IM |
Affiliation:
|
Laboratory of Histology, Faculty of Medicine and Pharmacy, University of Mons, Mons, Belgium. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Binding Sites Galectin 1 / metabolism* Galectin 3 / metabolism* Immunohistochemistry Kidney Tubules / metabolism* Male Rats Rats, Wistar Regeneration Reperfusion Injury / metabolism*, physiopathology |
| Chemical | |
Reg. No./Substance:
|
0/Galectin 1; 0/Galectin 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Reduced UVB-induced corneal damage caused by reactive oxygen and nitrogen species and decreased chan...
Next Document: The relationship between pregnancy-associated plasma protein-A (PAPP-A) and human intervertebral dis...