Document Detail


Immunohistochemical localization of cyclic guanosine monophosphate (cGMP) on mouse fetal chromosomes.
MedLine Citation:
PMID:  6174567     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In previous immunohistochemistry studies, cyclic guanosine monophosphate (cGMP) has been found in polytene chromosomes of D. melanogaster, cGMP has not been found in mammalian metaphase chromosomes, but this could be due to loss of cGMP during staining. Thus the effect of different fixation techniques on the immunohistochemically detectable cGMP associated with metaphase chromosomes from mouse fetal tissue was examined. In chromosomes from cells fixed in 2% formalin, or unfixed cells dropped on slides preheated to 60 degrees C, there was diffuse cGMP staining. When cells were fixed in methanol:glacial acetic acid, 3:1, no chromosomal cGMP immunofluorescence was observed, whereas chromosomes from cells fixed in methanol:glacial acetic acid, 6:1, had different patterns of cGMP immunofluorescence depending on the temperature of the slides onto which the fixed cells were dropped. On slides prechilled to 4 degrees C, cGMP immunofluorescence outlined the chromosomes; on room temperature slides, faint chromosomal cGMP staining was observed, and on slides preheated to 68 degrees C or room temperature slides blown dry with hot air, the chromosomes had more intense diffuse cGMP immunofluorescence or distinct symmetrical bands of cGMP immunofluorescence. We have demonstrated the presence of cGMP in mammalian metaphase chromosomes. The different patterns of cGMP immunofluorescence observed may reflect variable preservation of chromosomal proteins that have binding sites for cGMP.
Authors:
S M Tucci; E M Rosenberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  30     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1982 Apr 
Date Detail:
Created Date:  1982-05-21     Completed Date:  1982-05-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  343-50     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromosomes / analysis*,  ultrastructure
Cyclic GMP / analysis*
Female
Fetus
Fluorescent Antibody Technique
Metaphase
Mice
Mice, Inbred ICR
Pregnancy
Staining and Labeling
Grant Support
ID/Acronym/Agency:
R01-CA22690/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
7665-99-8/Cyclic GMP

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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