Document Detail


Immunohistochemical localization of Clara cell secretory proteins (CC10-CC26) and Annexin-1 protein in rat major salivary glands.
MedLine Citation:
PMID:  19438769     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The oral cavity is continuously bathed by saliva secreted by the major and minor salivary glands. Saliva is the first biological medium to confront external materials that are taken into the body as part of food or drink or inhaled volatile substances, and it contributes to the first line of oral defence. In humans, it has been shown that sputum and a variety of biological fluids contain Clara cell secretory proteins (CC10-CC26). Various studies of the respiratory apparatus have suggested their protective effect against inflammatory response and oxidative stress. Recently, CC10 deficiency has been related to the protein Annexin-1 (ANXA1), which has immunomodulatory and anti-inflammatory properties. Considering the defensive role of both Clara cell secretory proteins and ANXA1 in the respiratory apparatus, and the importance of salivary gland secretion in the first line of oral defence, we decided to evaluate the expression of CC10, CC26 and ANXA1 proteins in rat major salivary glands using immunohistochemistry. CC10 expression was found only in the ductal component of the sublingual gland. Parotid and submandibular glands consistently lacked CC10 immunoreactivity. In the parotid gland, both acinar and ductal cells were always CC26-negative, whereas in the submandibular gland, immunostaining was localized in the ductal component and in the periodic acid Schiff (PAS)-positive area. In the sublingual gland, ductal cells were always positive. Acinar cells were not immunostained at all. ANXA1 was expressed in ductal cells in all three major glands. In parotid and sublingual glands, acinar cells were negative. In submandibular glands, immunostaining was present in the mucous PAS-positive portion, whereas serous acinar cells were consistently negative. The existence of some CC10-CC26-ANXA1-positive cells in rat salivary glandular tissue is an interesting preliminary finding which could support the hypothesis, suggested for airway tissue, that these proteins have a defensive and protective role. Protein expression heterogeneity in the different portions of the glands could be an important clue in further investigations of their role.
Authors:
Maria Paola Cecchini; Flavia Merigo; Mirko Cristofoletti; Francesco Osculati; Andrea Sbarbati
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of anatomy     Volume:  214     ISSN:  1469-7580     ISO Abbreviation:  J. Anat.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-14     Completed Date:  2010-05-11     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  0137162     Medline TA:  J Anat     Country:  England    
Other Details:
Languages:  eng     Pagination:  752-8     Citation Subset:  IM    
Affiliation:
Department of Morphological and Biomedical Sciences, Anatomy and Histology Section, Faculty of Medicine, University of Verona, Italy. mariapaola@anatomy.univr.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Annexin A1 / immunology*
Enzyme Inhibitors / immunology*
Female
Male
Rats
Rats, Wistar / immunology*
Salivary Glands / cytology,  immunology*
Uteroglobin / immunology*
Chemical
Reg. No./Substance:
0/Annexin A1; 0/Enzyme Inhibitors; 0/SCGB1A1 protein, human; 0/Scgb1a1 protein, rat; 9060-09-7/Uteroglobin
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