Document Detail


Immunohistochemical localisation of mGluR7 protein in the rodent and human cerebellar cortex using subtype specific antibodies.
MedLine Citation:
PMID:  9630572     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Metabotropic glutamate receptors (mGluRs) are a heterogeneous family of G protein coupled receptors that are linked to multiple second messenger systems to regulate neuronal excitability and synaptic transmission. To characterise the protein expression of the two mGluR7 receptor splice variants in human and rat cerebellar cortex, antibodies specific to mGluR7 were generated. Antibodies were raised against a glutathione-S-transferase fusion protein containing amino acid residues located in the extracellular domain common to both the human and rat mGluR7 splice variants. These antibodies specifically detected human mGluR7a in mammalian cells transfected with this receptor. In agreement with mGluR7 in situ hybridisation studies, immunohistochemistry performed at the light microscope level revealed that mGluR7 protein expression occurred most prominently in a particular population of nerve cells common to both the human and rat, located within the cerebellar cortex of gray matter contained within transverse folia. Moreover, strong mGluR7-like immunoreactivity was seen in Purkinje cell body cytoplasm of the Purkinje cell layer. In the most superficial cerebellar cortical layer, the molecular layer, immunostaining was observed in Purkinje cell associated proximal and distal dendritic trees. No detectable labelling was evident in intrinsic deep cerebellar nuclei known to contain GABAergic terminals of projecting Purkinje cell axons. These data are suggestive of a post-synaptic location of mGluR7 in this central nervous system structure. In the rodent, additional non-Purkinje cells thought to represent inhibitory interneurones were labelled at all levels in the molecular layer. mGluR7-like immunoreactivity was not associated with glial cells.
Authors:
T Phillips; A Makoff; E Murrison; M Mimmack; H Waldvogel; R Faull; S Rees; P Emson
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research. Molecular brain research     Volume:  57     ISSN:  0169-328X     ISO Abbreviation:  Brain Res. Mol. Brain Res.     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-08-17     Completed Date:  1998-08-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8908640     Medline TA:  Brain Res Mol Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  132-41     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Elsevier Science B.V. All rights reserved.
Affiliation:
Laboratory of Molecular Neuroscience, Department of Neurobiology, Babraham Institute, Cambridge CB2 4AT, UK. tim.phillips@bbsrc.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / isolation & purification
Antibody Specificity*
Blotting, Western
COS Cells / physiology
Cerebellar Cortex / chemistry*
Glutathione Transferase / analysis,  immunology
Humans
Immunohistochemistry
Rabbits
Rats
Rats, Wistar
Receptors, Metabotropic Glutamate / analysis*,  immunology*
Recombinant Fusion Proteins / analysis,  immunology
Chemical
Reg. No./Substance:
0/Antibodies; 0/Receptors, Metabotropic Glutamate; 0/Recombinant Fusion Proteins; 0/metabotropic glutamate receptor 7; EC 2.5.1.18/Glutathione Transferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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