Document Detail


Immunohistochemical investigation and northern blot analysis of c-erB-2 expression in normal, premalignant and malignant tissues of the corpus and cervix uteri.
MedLine Citation:
PMID:  1980167     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Seventy specimens of normal endometrium (n = 13) and cervix (n = 12), endometrial hyperplasia (n = 4), cervical dysplasia (n = 20), endometrial (n = 11) and cervical carcinoma (n = 8) and uterine metastases of mammary carcinomas (n = 2) have been analysed for c-erB-2 expression with immunohistochemistry employing a monoclonal anti ERBB-2 antibody and Northern-blot hybridization using single stranded RNA probes. In comparison with the c-erbB-2 mRNA expression level found in normal samples, two advanced and poorly differentiated endometrial adenocarcinomas (FIGO IV) and two ductal mammary carcinomas which had metastasized to the uterus, together with three carcinomas in situ of the cervix, showed c-erbB-2 enhanced transcription level. All other endometrial samples including adenomatous hyperplasia and nine endometrial carcinomas (FIGO I), and all other lesions of squamous epithelial origin displayed transcriptional activities at or below the baseline level. Immunohistochemical study of ERBB-2 protein expression showed staining in most samples, although different in distribution and intensity. Staining of endometrial glands was seen in unevenly distributed cells or cell clusters. In contrast, for endocervical glands, labelling was observed distinctly on basally located cells (reserve cells) and at the subapical side of luminal cells. Faint labelling of the basal cell layer was also observed in squamous epithelia. It was more pronounced in severe cervical dysplasia and carcinoma in situ. In carcinomas of glandular origin, dedifferentiation was accompanied by an increase in cytoplasmic labelling, whereas the intensity of staining was not related to differentiation in squamous cell carcinomas. While data derived from Northern blots are suggestive of c-erbB-2 overexpression to indicate an advanced and dedifferentiated state of tumours of glandular origin, staining with an anti-ERBB-2 antibody occurred in both normal and atypical squamous and glandular epithelia and may indicate regular proliferation and/or differentiation-associated events.
Authors:
C Brumm; A Rivière; C Wilckens; T Löning
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virchows Archiv. A, Pathological anatomy and histopathology     Volume:  417     ISSN:  0174-7398     ISO Abbreviation:  Virchows Arch A Pathol Anat Histopathol     Publication Date:  1990  
Date Detail:
Created Date:  1991-02-15     Completed Date:  1991-02-15     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8302198     Medline TA:  Virchows Arch A Pathol Anat Histopathol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  477-84     Citation Subset:  IM    
Affiliation:
Institute of Pathology, University of Hamburg, Federal Republic of Germany.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / metabolism*,  pathology
Blotting, Northern
Carcinoma, Squamous Cell / metabolism*,  pathology
Female
Humans
Immunohistochemistry
Precancerous Conditions / metabolism*,  pathology
Proto-Oncogene Proteins / metabolism*
Proto-Oncogenes
Receptor, erbB-2
Reference Values
Uterine Cervical Neoplasms / metabolism*,  pathology
Uterine Neoplasms / metabolism*,  pathology
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins; EC 2.7.10.1/Receptor, erbB-2

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