Document Detail


Immunohistochemical identification of rat adrenal cortical tissue in situ, in vitro, and in intracerebral adrenal grafts.
MedLine Citation:
PMID:  7687964     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have used a polyclonal antibody to the biosynthetic adrenocortical enzyme, 3-beta-hydroxysteroid dehydrogenase, to demonstrate rat adrenal cortical tissue in situ, in vitro, and in intracerebral adrenal suspension grafts. This technique provides a means of identifying the degree to which adrenal medulla grafts are in practice contaminated by adrenal cortical cells and allows analysis of the extent to which the presence of cortical cells influences the viability, differentiation, and functional efficacy of intracerebral adrenal grafts.
Authors:
R Barker; S Dunnett; J Fawcett
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental neurology     Volume:  122     ISSN:  0014-4886     ISO Abbreviation:  Exp. Neurol.     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-09-02     Completed Date:  1993-09-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370712     Medline TA:  Exp Neurol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  125-9     Citation Subset:  IM    
Affiliation:
MRC Cambridge Brain Repair Centre, University of Cambridge, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
3-Hydroxysteroid Dehydrogenases / metabolism
Adrenal Cortex / metabolism*,  transplantation*
Animals
Antibodies, Monoclonal
Brain / physiology*
Immunohistochemistry / methods
Rats
Staining and Labeling
Tyrosine 3-Monooxygenase / metabolism
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; EC 1.1.-/3-Hydroxysteroid Dehydrogenases; EC 1.14.16.2/Tyrosine 3-Monooxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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