Document Detail

Immunohistochemical and histochemical profile of Aschoff bodies in rheumatic carditis in excised left atrial appendages: an immunoperoxidase study in fresh and paraffin-embedded tissue.
MedLine Citation:
PMID:  1737671     Owner:  NLM     Status:  MEDLINE    
We have evaluated the nature of Aschoff cells within Aschoff bodies seen in 35 of 100 excised left atrial appendages from cases of rheumatic mitral stenosis who underwent closed mitral valvotomy. These were tested using a panel of monoclonal and polyclonal antisera by the indirect immunoperoxidase staining for leucocyte common antigen, macrophage, desmin, vimentin, alpha-1-antitrypsin, alpha-1-antichymotrypsin, lysozyme, acid phosphatase and nonspecific esterase. The Aschoff cell gave strong reactivity with monoclonal antisera to vimentin, macrophage and variable reaction with polyclonal antisera known to recognise macrophages/histiocytes in tissues, namely alpha-1-antitrypsin, alpha-1-antichymotrypsin and lysozyme. These were also strongly positive for acid phosphatase and nonspecific esterase. The Aschoff cell lacked affinity for desmin and only an occasional cell in 4 out of 20 and 6 out of 35 cases showed a weak reaction with myoglobin and leucocyte common antigen, respectively. Intense consistent reactivity with several histiocytic markers affirms the genesis of these cells from macrophages/histiocytes and not muscle cells; a controversy which must be laid to rest!
P Chopra; J Wanniang; A Sampath Kumar
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of cardiology     Volume:  34     ISSN:  0167-5273     ISO Abbreviation:  Int. J. Cardiol.     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-03-19     Completed Date:  1992-03-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8200291     Medline TA:  Int J Cardiol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  199-207     Citation Subset:  IM    
Department of Pathology, All India Institute of Medical Sciences, New Delhi.
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MeSH Terms
Antibodies, Monoclonal
Heart Atria / pathology
Histiocytes / pathology
Immunoenzyme Techniques
Macrophages / pathology
Mitral Valve Stenosis / pathology*
Paraffin Embedding
Rheumatic Heart Disease / pathology*
Rheumatic Nodule / etiology,  pathology*
Tissue Embedding
Reg. No./Substance:
0/Antibodies, Monoclonal
Comment In:
Int J Cardiol. 1992 Sep;36(3):371-2   [PMID:  1428275 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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