Document Detail


Immunohistochemical expression of neural tissue markers (neuron-specific enolase, glial fibrillary acidic protein, S100 protein) in ameloblastic fibrodentinoma: a comparative study with ameloblastic fibroma.
MedLine Citation:
PMID:  10972858     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Formalin-fixed paraffin-embedded sections of three cases of ameloblastic fibrodentinoma (AFD) were studied by the avidin-biotin-peroxidase complex method using antibodies against neuron-specific enolase (NSE), glial fibrillary acidic protein (GFAP) and S100 protein and the results were compared with those in ameloblastic fibroma (AF). A striking histopathological characteristic of AFD was the formation of abortive dentin with various degrees of maturation at the epithelial-mesenchymal tissue interface. Central cells of enamel organ-like epithelia with various stages of abortive dentin induction in AFD were generally positive for NSE. Dental lamina-like epithelial cells also showed positive staining in some areas. No cells were positive for NSE in AF. Positive staining for GFAP was observed in the juxta-epithelial mesenchymal tissue of the formation stage of immature dentin with various numbers of entrapped cells in AFD, but GFAP staining was negative in other mesenchymal and epithelial tissues at other stages. In AF, no GFAP-positive cells were found. There were a few S100 protein-positive cells found in the foci of epithelial components in both AFD and AF. Mesenchymal cells showing a dendritic or spindle shape were positive for S100 protein in some areas of AFD and AF. Although such cells in the mesenchymal component of pigmented AFD were more numerous than in non-pigmented AFD and AF, their distribution pattern in the former condition was basically similar to that in the latter. Although the present results, obtained from conventional immunohistochemical procedures, do not directly reflect the expression of neural crest-derived cells in the dentinogenesis of AFD, such results do not disprove the possibility of the expression of neural proteins probably related to neural crest-derived cells in dentinogenesis under certain pathologic conditions in odontogenic mixed tumors. Such a phenomenon may also occur during dentinogenesis in other odontogenic mixed tumors and in normal tooth differentiation, but at an undetectable level.
Authors:
Y Takeda; H Sato; M Satoh; S I Nakamura; H Yamamoto
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pathology international     Volume:  50     ISSN:  1320-5463     ISO Abbreviation:  Pathol. Int.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-10-05     Completed Date:  2000-10-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9431380     Medline TA:  Pathol Int     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  610-5     Citation Subset:  IM    
Affiliation:
Department of Oral Pathology, School of Dentistry, Iwate Medical University, Morioka, Japan. ytakeda@iwate-med.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Adult
Ameloblastoma / metabolism*,  pathology,  surgery
Child
Child, Preschool
Dentin / pathology
Female
Glial Fibrillary Acidic Protein / metabolism*
Humans
Immunoenzyme Techniques
Male
Mandibular Neoplasms / metabolism*,  pathology,  surgery
Maxillary Neoplasms / metabolism*,  pathology,  surgery
Neoplasm Proteins / metabolism
Odontoma / metabolism*,  pathology,  surgery
Phosphopyruvate Hydratase / metabolism*
S100 Proteins / metabolism*
Tumor Markers, Biological / metabolism
Chemical
Reg. No./Substance:
0/Glial Fibrillary Acidic Protein; 0/Neoplasm Proteins; 0/S100 Proteins; 0/Tumor Markers, Biological; EC 4.2.1.11/Phosphopyruvate Hydratase

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