Document Detail

Immunohistochemical expression of angiogenic cytokines in childhood Hodgkin lymphoma.
MedLine Citation:
PMID:  18207652     Owner:  NLM     Status:  MEDLINE    
This immunohistochemical study was carried out to evaluate the role of vascular endothelial growth factor (VEGF), matrix metalloproteinase-2 and -9 (MMP-2 and MMP-9), their inhibitor (tissue inhibitor of metalloproteinase-1, TIMP-1), and microvessel density (MVD) in the clinicopathologic behavior of childhood Hodgkin's lymphoma (HL). Paraffin-embedded histologic sections from 15 children with HL were immunohistochemically stained with MMP-2, MMP-9, TIMP-1, VEGF, and CD31 antibodies to investigate the correlation between the expression of these markers and the clinicopathologic characteristics of HL. Expression of MMP-2 and VEGF in Hodgkin and Reed-Sternberg cells (HRS) was more frequent in nodular sclerosis than in other subtypes (p=0.07 and 0.08, respectively). None of the study parameters in HRS cell were associated with age, sex, disease stage, extranodal disease, and the occurrence of bulky tumor. There was a trend toward advanced stage in negative TIMP-1 staining in HRS cells (p=0.06). In reactive lymphocytes, MMP-2 expression was correlated with MVD (r=0.68, p=0.005), and MMP-9 expression was correlated with B symptoms (p=0.003). Also, low TIMP-1 expression in reactive lymphocytes was frequently found in patients with advanced stage (p=0.048). There was a positive correlation with the ratio of MMP-2 expression in reactive lymphocytes and MVD (r=0.68, p=0.005). Expression of MMP-9 in reactive lymphocytes was correlated with MVD without statistical significance (r=0.487, p=0.06). Our results suggest that, as in many solid tumors, angiogenesis and angiogenic factors may play an important role in childhood HL. Larger series of patients are needed to determine the prognostic value of angiogenesis in childhood HL.
Elvan Caglar Citak; Aynur Oguz; Ceyda Karadeniz; Nalan Akyurek
Publication Detail:
Type:  Journal Article     Date:  2008-01-18
Journal Detail:
Title:  Pathology, research and practice     Volume:  204     ISSN:  0344-0338     ISO Abbreviation:  Pathol. Res. Pract.     Publication Date:  2008  
Date Detail:
Created Date:  2008-03-03     Completed Date:  2008-05-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7806109     Medline TA:  Pathol Res Pract     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  89-96     Citation Subset:  IM    
Department of Pediatric Oncology, Faculty of Medicine, Gazi University, Huseyin Onat Sokak, No. 15/13, Asagiayranci, Ankara 06510, Turkey.
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MeSH Terms
Angiogenic Proteins / analysis*
Antibodies, Monoclonal
Antigens, CD31 / analysis
Child, Preschool
Hodgkin Disease / immunology,  metabolism*,  mortality,  pathology,  therapy
Kaplan-Meiers Estimate
Lymphocytes / chemistry
Matrix Metalloproteinase 2 / analysis
Matrix Metalloproteinase 9 / analysis
Microcirculation / immunology
Neoplasm Staging
Neovascularization, Pathologic / immunology,  metabolism*,  mortality,  pathology,  therapy
Reed-Sternberg Cells / chemistry
Tissue Inhibitor of Metalloproteinase-1 / analysis
Vascular Endothelial Growth Factor A / analysis
Reg. No./Substance:
0/Angiogenic Proteins; 0/Antibodies, Monoclonal; 0/Antigens, CD31; 0/Cytokines; 0/Tissue Inhibitor of Metalloproteinase-1; 0/VEGFA protein, human; 0/Vascular Endothelial Growth Factor A; EC protein, human; EC Metalloproteinase 2; EC Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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