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Immunohistochemical evaluation of type IV collagen and laminin-332 γ2 chain expression in well-differentiated oral squamous cell carcinoma and oral verrucous carcinoma: a new recommended cut-off.
MedLine Citation:
PMID:  21158930     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
J Oral Pathol Med (2011) 40: 167-173 Background:  Invasion and metastasis are two characteristics of malignant tumors, which perform by proteolytic destruction of the components of basement membrane (BM) and cell migration. The aim of this study was to evaluate the immunohistochemical (IHC) assessment of type IV collagen and laminin-332 γ2 (Ln-332 γ2) chain expression in well-differentiated oral squamous cell carcinoma (OSCC) and oral verrucous carcinoma (OVC), because these two lesions have same histopathologic findings whereas they have different biological behaviors. Methods:  Destruction of BM and cell migration were evaluated by IHC in 15 cases of epithelial hyperplasia with no dysplasia (A group), 15 cases of OVC (B group) and 15 cases of well-differentiated OSCC (C group). Results:  There was a significant difference in type IV collagen immunohistochemical staining between three groups, but there were no significant differences between B and C groups. Expression of Ln-332 γ2 chain was not detected in A group. Ln-332 γ2 chain labeling index had significantly difference between B and C groups. The number of Ln-332 γ2 chain immunostaining positive cells was less than 5% in B group and over than 5% in C group which there were significantly differences between these two groups. Conclusions:  Isolated immunohistochemical study of type IV collagen does not clearly define that a lesion is invasive or non-invasive and evaluation of Ln-332 γ2 chain expression (cut-off 5%) may be useful as a marker for description of biological differences and diagnosis of OVC from well-differentiated OSCC, especially in doubtful cases.
Authors:
Massoumeh Zargaran; Nosratollah Eshghyar; Pejman Bakianian Vaziri; Hamed Mortazavi
Publication Detail:
Type:  Journal Article     Date:  2010-12-15
Journal Detail:
Title:  Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology     Volume:  40     ISSN:  1600-0714     ISO Abbreviation:  J. Oral Pathol. Med.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8911934     Medline TA:  J Oral Pathol Med     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  167-73     Citation Subset:  D; IM    
Copyright Information:
© 2010 John Wiley & Sons A/S.
Affiliation:
Dental Research Center, Department of Oral and Maxillofacial Pathology, Dental School, Hamadan University of Medical Sciences Hamadan, Iran Department of Oral and Maxillofacial Pathology, Dental School, Tehran University of Medical Sciences, Tehran, Iran Department of Oral Medicine, Dental School, Shahed University of Medical Sciences, Tehran, Iran Department of Oral Medicine, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran.
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