Document Detail

Immunohistochemical evaluation of three nitric oxide synthase isoforms in human saphenous vein exposed to different degrees of distension pressures.
MedLine Citation:
PMID:  20060328     Owner:  NLM     Status:  MEDLINE    
The effect of short duration and different degrees of distension pressures was investigated by means of immunohistochemistry of the three nitric oxide synthase isoforms in the human saphenous vein conventionally harvested from 20 patients submitted to coronary artery bypass graft. The human saphenous vein distal portion was divided into four segments, each one allocated to a different group. In Group I (control group), the human saphenous vein segment was not exposed to distension pressure. In Groups II, III, and IV, the human saphenous vein segment was exposed to 100, 200, and 300 mmHg of distension pressure, respectively. The distension pressures were applied and maintained with Krebs solution for 15 s. The human saphenous vein of the control group presented endothelial nitric oxide synthase and neuronal nitric oxide synthase in both endothelial and smooth muscle cells, while the inducible nitric oxide synthase appeared predominantly in the medial layer. Neither 100 nor 200 mmHg of pressurization affected the immunostaining of any nitric oxide synthase isoform. However, the human saphenous vein segments exposed to 300 mmHg of distension pressure showed a reduction in endothelial nitric oxide synthase content in the endothelium, but not in the tunica media. This lower endothelial nitric oxide synthase immunostaining in the intimal cells was associated with endothelial denudation. Therefore, we conclude that care should be taken when handling the human saphenous vein since just a few seconds of distension pressure above the normal systemic pressure can be sufficient to disrupt the endothelium reducing the amount of endothelial nitric oxide synthase and impairing the graft quality.
Fernanda Viaro; Verena Kise Capellini; Andrea Carla Celotto; Carlos Gilberto Carlotti; Alfredo José Rodrigues; Graziela Saraiva Reis; Viviane dos Santos Augusto; Paulo Roberto Barbosa Evora
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-08
Journal Detail:
Title:  Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology     Volume:  19     ISSN:  1879-1336     ISO Abbreviation:  Cardiovasc. Pathol.     Publication Date:    2010 Nov-Dec
Date Detail:
Created Date:  2010-11-15     Completed Date:  2011-02-25     Revised Date:  2011-10-27    
Medline Journal Info:
Nlm Unique ID:  9212060     Medline TA:  Cardiovasc Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e211-20     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Department of Surgery and Anatomy, Ribeirão Preto Medical School, São Paulo University, Ribeirão Preto, São Paulo, Brazil.
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MeSH Terms
Coronary Artery Bypass
Endothelial Cells / enzymology
Myocytes, Smooth Muscle / enzymology
Nitric Oxide Synthase Type I / analysis*
Nitric Oxide Synthase Type II / analysis*
Nitric Oxide Synthase Type III / analysis*
Saphenous Vein / enzymology*,  surgery
Tissue and Organ Harvesting
Reg. No./Substance:
EC protein, human; EC protein, human; EC protein, human; EC Oxide Synthase Type I; EC Oxide Synthase Type II; EC Oxide Synthase Type III

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