Document Detail

Immunohistochemical evaluation of cell proliferation and apoptosis markers in ovaries and uterus of tamoxifen-treated rats.
MedLine Citation:
PMID:  17466037     Owner:  NLM     Status:  MEDLINE    
The aim of the study was to evaluate the immunohistochemical expression of cell proliferation and apoptosis markers in the ovaries and uterus of tamoxifen-treated rats. Twelve rats (150-200 g) were divided into two equal groups. The study group received daily intraperitoneal injections of tamoxifen dissolved in 5% dimethyl sulfoxide (n= 6). The control group received only the vehicle (n= 6). The rats were sacrificed at the 20th day of injection and were perfused. The ovaries and uterus of the rats were extracted. The sections were immunohistochemically stained with cell proliferation marker Ki-67 and the apoptosis markers PTEN and CD95. The expressions of the markers were quantified by a semiquantitative H-score method in myometrium, endometrial glands, ovarian surface epithelium, ovarian follicles, corpus luteum, and ovarian stroma separately. The mean H-scores of CD95 and PTEN obtained from myometrium, glandular endometrium, ovarian surface epithelium, ovarian follicles, corpus luteum, and ovarian stroma did not show significant difference between the study and the control groups. Proliferative index (Ki-67) of endometrial glands was significantly higher in the study group than in the control group (P < 0.05). In addition, proliferative index (Ki-67) of corpus luteum was significantly higher in the study group than in the control group (P < 0.05). Tamoxifen treatment has a potential to stimulate the cell proliferation of endometrial glands and corpus luteum in tamoxifen-treated rats. Apoptosis markers of PTEN and CD95 did not demonstrate significant difference after the tamoxifen treatment.
T Cirpan; M C Terek; M Ulukus; E C Ulukus; L Akman; L Kanit
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Publication Detail:
Type:  Journal Article     Date:  2007-04-26
Journal Detail:
Title:  International journal of gynecological cancer : official journal of the International Gynecological Cancer Society     Volume:  18     ISSN:  1525-1438     ISO Abbreviation:  Int. J. Gynecol. Cancer     Publication Date:    2008 Jan-Feb
Date Detail:
Created Date:  2008-01-14     Completed Date:  2008-03-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9111626     Medline TA:  Int J Gynecol Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  141-5     Citation Subset:  IM    
Department of Obstetrics and Gynecology, Ege University Faculty of Medicine, Izmir, Turkey.
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MeSH Terms
Antigens, CD95 / metabolism
Antineoplastic Agents, Hormonal / pharmacology*
Apoptosis / drug effects*,  physiology
Biological Markers / metabolism*
Cell Proliferation / drug effects*
Endometrium / drug effects,  metabolism
Ki-67 Antigen / metabolism
Ovary / drug effects*,  metabolism
PTEN Phosphohydrolase / metabolism
Tamoxifen / pharmacology*
Uterus / drug effects*,  metabolism
Reg. No./Substance:
0/Antigens, CD95; 0/Antineoplastic Agents, Hormonal; 0/Biological Markers; 0/Ki-67 Antigen; 10540-29-1/Tamoxifen; EC protein, rat; EC Phosphohydrolase

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