Document Detail


Immunohistochemical differentiation of eosinophilic heart diseases using antibodies against eosinophil activation markers.
MedLine Citation:
PMID:  15656891     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: Eosinophilic heart syndromes are rare in Western countries and include endocarditis parietalis fibroplastica (EPF) and hypersensitivity myocarditis (HM). There are striking differences in natural history and morphological findings. Since diagnosis can be difficult when analysing small myocardial biopsies lacking the characteristic histological features, we studied a set of immunohistochemical markers in order to characterize the activation status of the infiltrating eosinophils to distinguish between these two entities. METHODS AND RESULTS: This study is based on the investigation of seven explanted hearts and one left ventricular specimen collected during implantation of a left ventricular assist device from a total of seven patients with HM. Also investigated were three right and three left ventricular specimens from five patients with EPF. We used antibodies (Ab) against EG1, and EG2, CD44, and CD69 which have been described as markers to distinguish between resting and activated eosinophils. The EG1 to EG2 ratio of eosinophils and the immunoreactivity against CD44 showed no differences between the two entities. However, eosinophils in the EPF were completely negative for CD69, whereas eosinophils reacted positively within the HM group. CONCLUSION: The immunohistochemical investigation of eosinophilic heart diseases using antibodies against CD69 can be a useful tool to distinguish between hypersensitivity myocarditis and endocarditis parietalis fibroplastica.
Authors:
F Grabellus; G Mall; P A Schnabel; H Wieneke; U Pfeifer; C Kersting; K J Schmitz; J Wohlschläger; H H Sigusch; E Bierhoff; M Kamler; H-H Scheld; H A Baba
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Histopathology     Volume:  46     ISSN:  0309-0167     ISO Abbreviation:  Histopathology     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-01-19     Completed Date:  2005-05-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7704136     Medline TA:  Histopathology     Country:  England    
Other Details:
Languages:  eng     Pagination:  89-97     Citation Subset:  IM    
Affiliation:
Institute of Pathology, University Hospital of Essen, Essen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Aged
Animals
Antibodies, Monoclonal / immunology
Antigens, CD / immunology
Antigens, Differentiation, T-Lymphocyte / immunology
Diagnosis, Differential
Endocarditis / diagnosis,  immunology,  pathology
Eosinophils / immunology*,  pathology
Female
Heart Diseases / diagnosis,  immunology*,  pathology
Humans
Immunohistochemistry*
Male
Mice
Middle Aged
Myocarditis / diagnosis,  immunology,  pathology
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD; 0/Antigens, Differentiation, T-Lymphocyte; 0/CD69 antigen

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