Document Detail


Immunohistochemical diagnosis of pulmonary eosinophilic granuloma on lung biopsy.
MedLine Citation:
PMID:  2461275     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
S-100 protein immunostaining has been advocated to identify the characteristic Langerhans' cells in the histologic diagnosis of PEG. Reliable demonstration of an increased number of Langerhans' cells is essential in difficult biopsy cases, since occasional Langerhans' cells can be found in other pulmonary lesions. We examined the S-100 protein labeling pattern in three cases of PEG and in a variety of controls. Non-Langerhans' histiocytes were labeled for lysozyme antigen on the same histologic sections using a combined ABC and PAP technique. This verified that the S-100 protein-negative histiocytes were indeed a separate population from the S-100 protein-positive histiocytes and did not represent Langerhans' cells which failed to label with antiserum to S-100 protein. This technique confirms the usefulness of S-100 protein staining in the diagnosis of PEG and offers a means to verify the reliability of the S-100 protein labeling in questionable cases.
Authors:
P T Cagle; C A Mattioli; L D Truong; S D Greenberg
Related Documents :
10402265 - A novel peroxisomal enzyme, d-3-hydroxyacyl-coa dehydratase/d-3-hydroxyacyl-coa dehydro...
2831325 - Cyclic amp-induced phosphorylation of 27.5-kda protein(s) in larval brains of normal an...
21319305 - From eosfp to mirisfp: structure-based development of advanced photoactivatable marker ...
3692925 - Golgi-like immunostaining of pituicytes and tanycytes positive for glial fibrillary aci...
2590205 - The effect of asparagine and adenine on the glutamine requirement for growth of human p...
9368755 - Cryo-electron microscopy reveals ordered domains in the immature hiv-1 particle.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chest     Volume:  94     ISSN:  0012-3692     ISO Abbreviation:  Chest     Publication Date:  1988 Dec 
Date Detail:
Created Date:  1989-01-12     Completed Date:  1989-01-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0231335     Medline TA:  Chest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1133-7     Citation Subset:  AIM; IM    
Affiliation:
Department of Pathology, Baylor College of Medicine, Houston.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Biopsy
Eosinophilic Granuloma / diagnosis*,  pathology
Granulocytes / pathology
Histiocytes / pathology
Humans
Immunohistochemistry
Langerhans Cells / pathology
Lung / pathology
Lung Diseases / diagnosis*,  pathology
Muramidase / diagnostic use
S100 Proteins / diagnostic use
Staining and Labeling
Chemical
Reg. No./Substance:
0/S100 Proteins; EC 3.2.1.17/Muramidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Simplified C-banding of mouse chromosomes.
Next Document:  Cytological evidence of defective template in the fragile X chromosome.