| Immunohistochemical and developmental studies to elucidate the mechanism of action of the snowdrop lectin on the rice brown planthopper, Nilaparvata lugens (Stal). | |
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MedLine Citation:
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PMID: 12769935 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Rice brown planthoppers (Nilaparvata lugens) were fed on artificial diet containing snowdrop lectin (Galanthus nivalis agglutinin; GNA), which has been shown to be toxic towards this insect pest. In addition to decreasing survival, the lectin affected development, reducing the growth rate of nymphs by approximately 50% when present at a concentration of 5.3&mgr;M. Immunolocalisation studies showed that lectin binding was concentrated on the luminal surface of the midgut epithelial cells within the planthopper, suggesting that GNA binds to cell surface carbohydrate moieties in the gut. Immunolabelling at a lower level was also observed in the fat bodies, the ovarioles, and throughout the haemolymph. These observations suggest that GNA is able to cross the midgut epithelial barrier, and pass into the insect's circulatory system, resulting in a systemic toxic effect. Electron microscope studies showed morphological changes in the midgut region of planthoppers fed on a toxic dose of GNA, with disruption of the microvilli brush border region. No significant proteolytic degradation of GNA was observed either in the gut or honeydew of planthoppers fed on lectin-containing diet. The presence of glycoproteins which bind GNA in the gut of the brown planthopper was confirmed using digoxigen-labeled lectins to probe blots of extracted gut polypeptides. |
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Authors:
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A M.R. Gatehouse; J A. Gatehouse; M Bharathi; J Spence; K S. Powell |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Journal of insect physiology Volume: 44 ISSN: 1879-1611 ISO Abbreviation: J. Insect Physiol. Publication Date: 1998 Jul |
Date Detail:
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Created Date: 2003-May-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985080R Medline TA: J Insect Physiol Country: - |
Other Details:
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Languages: ENG Pagination: 529-539 Citation Subset: - |
Affiliation:
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University of Durham, Department of Biological Sciences, South Road, Durham, UK |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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