Document Detail

Immunohistochemical determination of complement activation in joint tissues of patients with rheumatoid arthritis and osteoarthritis using neoantigen-specific monoclonal antibodies.
MedLine Citation:
PMID:  1285067     Owner:  NLM     Status:  MEDLINE    
Murine monoclonal antibodies specific for neoepitopes expressed by C9 incorporated into membrane attack complexes and by membrane-bound C3b and iC3b have been prepared and characterised. These reagents were used to determine the extent and locus of complement activation in synovial-tissues obtained from patients with rheumatoid arthritis and osteoarthritis. In the four rheumatoid arthritis patients there was extensive deposition of C3 activation products and C5b-9 complexes onto the synovial membrane and the pattern of deposition of both neoantigens in serial tissue sections was very similar. There was less extensive staining for C3 and, particularly, C9 neoepitopes on the apical surface of vessel endothelia. In two of four osteoarthritic patients a similar pattern of C3 and C9 neoepitope deposition was found; in the remaining patients no C5b-9 could be located. Synovial vessel walls, but not synovial cells, from both groups of patients stained extensively for the complement regulatory protein CD59. In synovial membranes from patients with osteoarthritis, C9 appeared to be present predominantly in SC5b-9 complexes whereas in rheumatoid arthritis patients no evidence of S-protein incorporation into membrane attack complexes could be demonstrated, suggesting that in rheumatoid arthritis there is damage to the synovial membrane as a result of complement activation and C5b-9 deposition.
P A Kemp; J H Spragg; J C Brown; B P Morgan; C A Gunn; P W Taylor
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical & laboratory immunology     Volume:  37     ISSN:  0141-2760     ISO Abbreviation:  J Clin Lab Immunol     Publication Date:  1992  
Date Detail:
Created Date:  1993-11-19     Completed Date:  1993-11-19     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  7808987     Medline TA:  J Clin Lab Immunol     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  147-62     Citation Subset:  IM    
Research and Preclinical Development, CIBA-Geigy Pharmaceuticals, West Sussex, UK.
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MeSH Terms
Aged, 80 and over
Antibodies, Monoclonal
Arthritis, Rheumatoid / immunology*
Complement Activation*
Complement C3b / metabolism
Complement C9 / metabolism
Complement Membrane Attack Complex / metabolism
Epitopes / metabolism
Middle Aged
Osteoarthritis / immunology*
Synovial Membrane / immunology*
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Complement C9; 0/Complement Membrane Attack Complex; 0/Epitopes; 80295-43-8/Complement C3b

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