Document Detail

Immunohistochemical detection of ras and myc oncogene expression in regenerating rat liver.
MedLine Citation:
PMID:  1570626     Owner:  NLM     Status:  MEDLINE    
The expression of ras and myc oncogene proteins was examined in formalin-fixed paraffin-embedded sections of male Sprague-Dawley rat liver at various times after liver regeneration was induced by either a necrogenic dose of CCl4 or a 2/3 partial hepatectomy. Antibody specific for these proteins and immunohistochemical (IHC) procedures were used to detect oncogene expression in the liver. Increased expression of both oncogene proteins was detected at times comparable to those reported by others using either Northern or Western blot methods. However, the IHC procedure provided additional information since oncogene expression was detected only in hepatocytes, mainly occupying liver lobule zones known to contain proliferating hepatocytes during regeneration. Also, ras and myc stain intensity of individual hepatocytes did not increase during regeneration. Rather, a greater number of hepatocytes were positive at several time points in the regeneration process. This suggested that the increased ras and myc expression detected in Northern and Western blot methods probably represents a greater number of hepatocytes expressing the oncogene proteins rather than increased expression by individual hepatocytes. Overall, this study provides additional information on the cells involved in the expression of the ras and myc oncogenes during regeneration and further substantiates their role in a normal physiological process.
R E Richmond; A B DeAngelo; F B Daniel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Toxicology letters     Volume:  60     ISSN:  0378-4274     ISO Abbreviation:  Toxicol. Lett.     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-05-26     Completed Date:  1992-05-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  119-29     Citation Subset:  IM    
Department of Biological Sciences, Northern Kentucky University, Highland Heights.
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MeSH Terms
Carbon Tetrachloride / toxicity
Gene Expression / drug effects
Liver / metabolism*,  pathology
Liver Regeneration / drug effects
Oncogene Protein p21(ras) / analysis*
Oncogene Protein p55(v-myc) / analysis*
Rats, Inbred Strains
Reg. No./Substance:
0/Oncogene Protein p55(v-myc); 56-23-5/Carbon Tetrachloride; EC Protein p21(ras)

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