Document Detail


Immunohistochemical detection of glucose transporters class I subfamily in the mouse, rat and human testis.
MedLine Citation:
PMID:  15007275     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A family of glucose transporters (GLUT) mediates the cellular uptake of glucose at the plasma membrane by facilitated diffusion. We investigated the presence of isoforms GLUT1-4 of class I subfamilies in different types of cells in the mouse, rat and human testis by indirect immunofluorescence technique. Immunocytochemical analyses demonstrated that GLUT1 was expressed in the rat testis, GLUT2 in the mouse and rat testis, GLUT3 in the mouse, rat and human testis and GLUT4 was not presented in the testis at all. A very intensive positive immunoreaction for GLUT3 was found in Sertoli cells, peritubular myoid cells, macrophage-like interstitial cells, testicular endothelial cells and early spermatocytes. GLUT3 positive cells were not found in the luminal part of Sertoli cells, spermatids or Leydig cells. The present results suggest that glucose uptake in different testicular cells is mediated by GLUT1, GLUT2 and GLUT3 and the GLUT3 was the prominent glucose transporter type in the testicular cells.
Authors:
Kersti Kokk; Esko Veräjänkorva; Xiao-Ke Wu; Helle Tapfer; Elle Põldoja; Pasi Pöllänen
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medicina (Kaunas, Lithuania)     Volume:  40     ISSN:  1648-9144     ISO Abbreviation:  Medicina (Kaunas)     Publication Date:  2004  
Date Detail:
Created Date:  2004-03-09     Completed Date:  2004-10-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9425208     Medline TA:  Medicina (Kaunas)     Country:  Lithuania    
Other Details:
Languages:  eng     Pagination:  156-60     Citation Subset:  IM    
Affiliation:
Department of Anatomy, University of Tartu, Biomedicum, Ravila 19, 50411 Tartu, Estonia. kerstiko@ut.ee
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Membrane / metabolism
Endothelial Cells / metabolism
Fluorescent Antibody Technique, Indirect
Frozen Sections
Glucose / metabolism*
Glucose Transporter Type 1
Glucose Transporter Type 3
Glucose Transporter Type 4
Humans
Immunohistochemistry
Leydig Cells / metabolism
Male
Mice
Mice, Inbred BALB C
Monosaccharide Transport Proteins / analysis*,  metabolism
Muscle Proteins
Nerve Tissue Proteins
Rats
Rats, Wistar
Sertoli Cells / metabolism
Spermatids / metabolism
Spermatocytes / metabolism
Testis / cytology,  metabolism*
Tissue Distribution
Chemical
Reg. No./Substance:
0/Glucose Transporter Type 1; 0/Glucose Transporter Type 3; 0/Glucose Transporter Type 4; 0/Monosaccharide Transport Proteins; 0/Muscle Proteins; 0/Nerve Tissue Proteins; 0/SLC2A1 protein, human; 0/SLC2A3 protein, human; 0/SLC2A4 protein, human; 0/Slc2a1 protein, mouse; 0/Slc2a1 protein, rat; 0/Slc2a3 protein, mouse; 0/Slc2a3 protein, rat; 0/Slc2a4 protein, mouse; 0/Slc2a4 protein, rat; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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