Document Detail

Immunohistochemical demonstration of migration inhibitory factor (MIF) in experimental allergic contact dermatitis.
MedLine Citation:
PMID:  3280179     Owner:  NLM     Status:  MEDLINE    
The kinetics of appearance of MIF+ cells was investigated in experimental contact dermatitis using a monoclonal antibody (7D10) against murine MIF which was reacted with cryostat sections of tissues and detected by the indirect immunoperoxidase test. Four groups of BALB/c mice were investigated: (1) sensitized with 2,4-dinitrofluorobenzene (DNFB); (2) unsensitized controls; (3) tolerized; (4) unsensitized. A challenge dose of DNFB was applied to the ear of animals of groups 1-3 and of croton oil to those of group 4. Three phases could be distinguished in group 1: (a) an initial vascular and exudative reaction; (b) an early cellular phase; and (c) a late cellular phase. At zero time rarely any T lymphocytes (Lyt 1+; Lyt 2+) were seen in all four groups. Within less than 30 min venous endothelial cells became strongly MIF+. This was followed by an influx of monocytes/macrophages reaching a maximum of 72 h in group 1 and a slight peak at 12 h in groups 2 and 3. At 16-24 h in all groups the endothelial reaction weakened while many 7D10+ macrophages appeared in group 1. By double-labelling it was shown that lymphocytes were 7D10-. The influx of lymphocytes, part of which carried the T cell receptor, began at 12 h, reaching a maximum at 72 h in group 1. In groups 2 and 3 only a weak lymphocytic infiltrate developed which declined at 24 h. Group 4 developed an inflammatory reaction after the initial phase with similar kinetics as in group 1. The data suggest that an immune inflammatory reaction is preceded by a nonspecific reaction of the vascular endothelium and the mononuclear phagocytic system and that MIF is playing a central role in these events.
U Malorny; J Knop; G Burmeister; C Sorg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  71     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  1988 Jan 
Date Detail:
Created Date:  1988-05-02     Completed Date:  1988-05-02     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  164-70     Citation Subset:  IM    
Department of Experimental Dermatology, University of Münster, FRG.
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MeSH Terms
Antibodies, Monoclonal / diagnostic use
Dermatitis, Contact / etiology,  immunology*
Dinitrofluorobenzene / immunology
Dinitrophenols / analysis
Immunoenzyme Techniques
Macrophage Migration-Inhibitory Factors / analysis*
Mice, Inbred BALB C
Rats, Inbred Strains
Skin / immunology
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Dinitrophenols; 0/Macrophage Migration-Inhibitory Factors; 70-34-8/Dinitrofluorobenzene

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