Document Detail

Immunohistochemical demonstration of carcinogen-DNA adducts in tissues of rats given 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP): detection in paraffin-embedded sections and tissue distribution.
MedLine Citation:
PMID:  9766658     Owner:  NLM     Status:  MEDLINE    
A polyclonal antibody against 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)-DNA adducts was raised for their immunohistochemical demonstration in paraffin-embedded sections. Specificity of this antibody was confirmed by competitive ELISA. Positive signals were immunohistochemically detected in acetone-fixed but not in formalin- or ethanol-fixed sections from F344 rats treated by gavage with a single dose of PhIP at 37.5-300 mg/kg and killed at 1, 2, and 7 days thereafter. Dose-dependent positive staining was observed in almost all organs of both sexes, including the colon, prostate, and mammary gland but largely independent of the tumor response. Repair activity, judged by disappearance of adducts with time, differed according to the organ or cell type. One exception was hepatocytes, the liver incidentally being a nontarget organ. The results suggest that the generated antibody is applicable for detection of cells targeted by PhIP in paraffin-embedded sections and also for the investigation of the mechanisms of PhIP-carcinogenesis.
S Takahashi; S Tamano; M Hirose; N Kimoto; Y Ikeda; M Sakakibara; M Tada; F F Kadlubar; N Ito; T Shirai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer research     Volume:  58     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1998-11-03     Completed Date:  1998-11-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4307-13     Citation Subset:  IM    
First Department of Pathology, Nagoya City University Medical School, Nagoya, Japan.
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MeSH Terms
Administration, Oral
Carcinogens / administration & dosage,  pharmacokinetics*
Colon / cytology,  metabolism
Cross Reactions
DNA Adducts / analysis*
Enzyme-Linked Immunosorbent Assay
Imidazoles / administration & dosage,  pharmacokinetics*
Mammary Glands, Animal / cytology,  metabolism
Neoplasms, Experimental / chemically induced*,  pathology
Prostate / cytology,  metabolism
Rats, Inbred F344
Tissue Distribution
Reg. No./Substance:
0/Carcinogens; 0/DNA Adducts; 0/Imidazoles; 105650-23-5/2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine

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