Document Detail

Immunohistochemical demonstration of 14-3-3 sigma protein in normal human tissues and lung cancers, and the preponderance of its strong expression in epithelial cells of squamous cell lineage.
MedLine Citation:
PMID:  12787309     Owner:  NLM     Status:  MEDLINE    
In order to confirm 14-3-3 sigma (sigma) protein distribution in human tissues, immunohistochemistry was performed using various paraffin-embedded human tissues. In normal human tissues, the strongest immunoreactivity for 14-3-3sigma protein was observed in squamous epithelia at various sites, followed by basal cells of the trachea, bronchus and basal or myoepithelial cells of various glands. Moderate to weak 14-3-3sigma immunoreactivity was seen in the epithelial cells of the alimentary tract, gall bladder, urinary tract and endometrium. In the lung, 14-3-3sigma immunoreactivity was also observed in hyperplastic type II alveolar cells and metaplastic squamous cells. Immunohistochemical study using non-small-cell lung cancers revealed that 14-3-3sigma immunoreactivity was stronger in squamous cell carcinomas than in adenocarcinomas. The present study revealed that 14-3-3sigma expression was exclusively present in various epithelial cells and had a tendency to be stronger in cells destined for squamous epithelium or differentiating toward squamous cells in human normal and neoplastic cells.
Takashi Nakajima; Hanako Shimooka; Peng Weixa; Atsuki Segawa; Atsushi Motegi; Zhang Jian; Norihiko Masuda; Munenori Ide; Takaaki Sano; Tetsunari Oyama; Hiroe Tsukagoshi; Kozue Hamanaka; Masahiro Maeda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pathology international     Volume:  53     ISSN:  1320-5463     ISO Abbreviation:  Pathol. Int.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-06-05     Completed Date:  2004-03-16     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9431380     Medline TA:  Pathol Int     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  353-60     Citation Subset:  IM    
Second Department of Pathology and The First Department of Surgery, Gunma University School of Medicine, and Immuno-Biological Laboratories Co Ltd, Gunma, Japan.
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MeSH Terms
Adenocarcinoma / metabolism*,  pathology
Blotting, Western
Carcinoma, Squamous Cell / metabolism*,  pathology
Cell Count
Cell Lineage
Exonucleases / metabolism*
Immunoenzyme Techniques
Lung Neoplasms / metabolism*,  pathology
Neoplasm Proteins / metabolism*
Tissue Distribution
Tumor Markers, Biological / metabolism*
Reg. No./Substance:
0/Neoplasm Proteins; 0/Tumor Markers, Biological; EC 3.1.-/Exonucleases; EC 3.1.-/SFN protein, human

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