Document Detail

Immunohistochemical characterization of HSP, alpha-MSH, Merkel cells and neuronal markers in acute UV dermatitis and acute contact dermatitis in vivo.
MedLine Citation:
PMID:  9372313     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To study the immunoneurocrine network in inflammatory dermatoses, we investigated histochemically acute UV and acute contact dermatitis. METHODS: Antibodies were applied to frozen and paraffin specimens of human skin after irradiation (n = 10), to positive patch tests (n = 10) and controls (n = 10) against: HSP 70, 72, 27, neuronal polypeptides (alpha-MSH, NSE, bombesin, PGP 9.5, NGF, NGF-R) and intermediate filaments (peripherin, NF 200, CK 19, 20). RESULTS: HSPs and alpha-MSH were upregulated in UV dermatitis in the epidermis compared to contact dermatitis and normal skin. Sunburn cells did not express HSPs or alpha-MSH in UV dermatitis. Neuronal markers and HSP 27 labeled more nerve fibers in UV than in contact dermatitis, except the increased staining for NGF, NGF-R and alpha-MSH in nerve fibers in contact dermatitis. In UV dermatitis, 50% of Merkel cells were suprabasal, but in contact dermatitis, basal, rounded and reduced in number. CONCLUSIONS: Merkel cells, HSPs and markers of neuroinflammation are of different importance in UV and contact dermatitis in vivo.
C Bayerl; J Lauk; I Moll; E G Jung
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Inflammation research : official journal of the European Histamine Research Society ... [et al.]     Volume:  46     ISSN:  1023-3830     ISO Abbreviation:  Inflamm. Res.     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1997-12-23     Completed Date:  1997-12-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9508160     Medline TA:  Inflamm Res     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  409-11     Citation Subset:  IM    
Department of Dermatology, Mannheim Medical School, University of Heidelberg, Germany.
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MeSH Terms
Dermatitis / etiology*,  metabolism,  pathology
Dermatitis, Contact / metabolism,  pathology
Epidermis / metabolism,  pathology
Heat-Shock Proteins / analysis*
Keratinocytes / chemistry
Merkel Cells / pathology*
Nerve Growth Factors / analysis
Neurons / pathology*
Receptors, Nerve Growth Factor / analysis
Skin / metabolism,  pathology
Ultraviolet Rays*
alpha-MSH / analysis*
Reg. No./Substance:
0/Heat-Shock Proteins; 0/Nerve Growth Factors; 0/Receptors, Nerve Growth Factor; 581-05-5/alpha-MSH

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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