| Immunohistochemical and biochemical analysis of laminin in neonatal rat first molars. | |
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MedLine Citation:
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PMID: 9476430 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rabbit polyclonal antibody against mouse EHS laminin was used to investigate the distribution and composition of laminin in the rat first molar tooth germ. Immunohistochemical analysis showed that laminin is expressed in the inner and outer epithelia of the enamel organ and in small blood vessels in the dental papilla and strellate reticulum. Immunoblots revealed that tooth germ laminin differs from EHS laminin. Tooth germ laminin contains beta chains, while the alpha 1 chain is substituted by a 300-kDa chain. Two-dimensional electrophoresis analysis of tooth germ extract showed that beta chains appeared as four spots with approximate pI values of 6.6, 7.5, 7.8 and 8.5. These results indicate that more than-one type of laminin isoform is present in the first molar tooth germ. Additionally, we have shown that despite the early degradation of tooth germ basement membrane, the laminin molecule is still intact at the time of birth. |
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Authors:
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R Della Coletta; S S Veiga; S R Line |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of Nihon University School of Dentistry Volume: 39 ISSN: 0029-0432 ISO Abbreviation: J Nihon Univ Sch Dent Publication Date: 1997 Dec |
Date Detail:
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Created Date: 1998-03-10 Completed Date: 1998-03-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7509209 Medline TA: J Nihon Univ Sch Dent Country: JAPAN |
Other Details:
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Languages: eng Pagination: 176-81 Citation Subset: D; IM |
Affiliation:
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Discipline of Oral Pathology, Faculty of Odontology of Piracicaba-UNICAMP, Piracicaba-SP, Brazil. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Antibodies / diagnostic use Basement Membrane / ultrastructure Blotting, Western Capillaries / ultrastructure Dental Papilla / blood supply, ultrastructure Electrophoresis, Gel, Two-Dimensional Enamel Organ / ultrastructure Epithelium / ultrastructure Immunoblotting Immunohistochemistry Isomerism Laminin / analysis*, chemistry, classification Mice Molar Precipitin Tests Rabbits Rats Rats, Wistar Tooth Germ / ultrastructure* Tooth, Deciduous / ultrastructure* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Laminin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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