Document Detail


Immunohistochemical and biochemical analysis of laminin in neonatal rat first molars.
MedLine Citation:
PMID:  9476430     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rabbit polyclonal antibody against mouse EHS laminin was used to investigate the distribution and composition of laminin in the rat first molar tooth germ. Immunohistochemical analysis showed that laminin is expressed in the inner and outer epithelia of the enamel organ and in small blood vessels in the dental papilla and strellate reticulum. Immunoblots revealed that tooth germ laminin differs from EHS laminin. Tooth germ laminin contains beta chains, while the alpha 1 chain is substituted by a 300-kDa chain. Two-dimensional electrophoresis analysis of tooth germ extract showed that beta chains appeared as four spots with approximate pI values of 6.6, 7.5, 7.8 and 8.5. These results indicate that more than-one type of laminin isoform is present in the first molar tooth germ. Additionally, we have shown that despite the early degradation of tooth germ basement membrane, the laminin molecule is still intact at the time of birth.
Authors:
R Della Coletta; S S Veiga; S R Line
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of Nihon University School of Dentistry     Volume:  39     ISSN:  0029-0432     ISO Abbreviation:  J Nihon Univ Sch Dent     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-03-10     Completed Date:  1998-03-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7509209     Medline TA:  J Nihon Univ Sch Dent     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  176-81     Citation Subset:  D; IM    
Affiliation:
Discipline of Oral Pathology, Faculty of Odontology of Piracicaba-UNICAMP, Piracicaba-SP, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Antibodies / diagnostic use
Basement Membrane / ultrastructure
Blotting, Western
Capillaries / ultrastructure
Dental Papilla / blood supply,  ultrastructure
Electrophoresis, Gel, Two-Dimensional
Enamel Organ / ultrastructure
Epithelium / ultrastructure
Immunoblotting
Immunohistochemistry
Isomerism
Laminin / analysis*,  chemistry,  classification
Mice
Molar
Precipitin Tests
Rabbits
Rats
Rats, Wistar
Tooth Germ / ultrastructure*
Tooth, Deciduous / ultrastructure*
Chemical
Reg. No./Substance:
0/Antibodies; 0/Laminin

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