Document Detail


Immunohistochemical assessment of the tumour-associated epitopes CD44v6 and E48 in tumour-free lymph nodes from patients with squamous cell carcinoma in the head-neck region.
MedLine Citation:
PMID:  11153607     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We examined immunohistochemically 370 tumour-free lymph nodes from 41 patients with a head and neck squamous cell carcinoma (HNSCC) to clarify whether the tumour-associated epitopes CD44v6 and E48 are suitable for adjuvant postoperative immunotherapy. All the positively immunostained cells found were single cells. CD44v6+ cells were found in 55% of the lymph nodes, with their numbers increasing in pN>0-patients (62%). Only pN>0-patients had abundant to massive CD44v6+ cells. A comparison with mononuclear cells in lymphatic tissue from control patients suggested a similarity with activated T-cells. In the 41 cancer patients there were significantly fewer lymph nodes with E48+ cells (11%), but the number of E48+ cells increased in pN> 1-patients (29%) with predominantly abundant E48+ cells. We conclude from the comparison with the epithelial marker EMA that the E48+ single cells are epithelial in origin. Only a specific E48 peptide sequence appears suitable for adjuvant immunotherapy in patients with head-neck tumours.
Authors:
E M Fabricius; M Guschmann; A Langford; B Hell; J Bier
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology     Volume:  20     ISSN:  0921-8912     ISO Abbreviation:  Anal Cell Pathol     Publication Date:  2000  
Date Detail:
Created Date:  2001-01-10     Completed Date:  2001-03-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8911016     Medline TA:  Anal Cell Pathol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  115-29     Citation Subset:  IM    
Affiliation:
Clinic for Maxillofacial Surgery, Medical Faculty of the Humboldt University of Berlin, Charité, Germany. eva-maria.fabricius@charite.de
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MeSH Terms
Descriptor/Qualifier:
Aged
Antigens, CD44 / analysis*
Antigens, Neoplasm / analysis
Carcinoma, Squamous Cell / immunology*,  pathology
Cell Adhesion Molecules / analysis*
Epithelial Cells / immunology
Epitopes / analysis
Female
Glycoproteins / analysis*
Head and Neck Neoplasms / immunology*,  pathology
Humans
Immunohistochemistry
Lymph Nodes / immunology
Male
Middle Aged
Chemical
Reg. No./Substance:
0/Antigens, CD44; 0/Antigens, Neoplasm; 0/Cell Adhesion Molecules; 0/Epitopes; 0/Glycoproteins; 0/LY6D protein, human

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