Document Detail

An immunohistochemical analysis of a rat model of proliferative vitreoretinopathy and a comparison of the expression of TGF-β and PDGF among the induction methods.
MedLine Citation:
PMID:  20846126     Owner:  NLM     Status:  MEDLINE    
Proliferative vitreoretinopathy (PVR) is a serious complication of retinal detachment surgery or ocular trauma. Our previous study indicated that intravitreal co-injection of retinal pigmented epithelial (RPE)-J cells and platelet-rich plasma (PRP) (not RPE-J cells or PRP alone) in Wistar rat eyes can successfully induce a model of PVR. But which cells are involved in this process and why different induction methods, intravitreal injection of RPE-J cells or/and PRP, induced a different situation remain to be unknown. In this study, immunohistochemistry was performed to identify the main cell types involved in this process. The expression levels of transforming growth factor (TGF)-β2, platelet-derived growth factor (PDGF)-AA and PDGF-BB were tested using enzyme-linked immunosorbent assay (ELISA). The results showed that RPE cells, glial cells, fibroblasts and macrophages took part in the pathogenesis of this model. The expression levels and durations of TGF-β2 and PDGF-BB partially explained the different results induced by the different induction methods. This provides an experimental proof for attenuation of the experimental PVR by targeting at a specific cells or growth factor.
Xiao-Zhi Zheng; Lian-Fang Du; Hui-Ping Wang
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bosnian journal of basic medical sciences / Udruženje basičnih mediciniskih znanosti = Association of Basic Medical Sciences     Volume:  10     ISSN:  1840-4812     ISO Abbreviation:  Bosn J Basic Med Sci     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-09-17     Completed Date:  2011-01-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101200947     Medline TA:  Bosn J Basic Med Sci     Country:  Bosnia and Hercegovina    
Other Details:
Languages:  eng     Pagination:  204-9     Citation Subset:  IM    
Department of Ultrasound, The Fourth Affiliated Hospital of Nantong University, The First People's Hospital of Yancheng, Yancheng 224006, Jiangsu Province, P.R.China.
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MeSH Terms
Blood Platelets / physiology
Cell Transplantation
Cells, Cultured
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Glial Fibrillary Acidic Protein / metabolism
Platelet-Derived Growth Factor / biosynthesis*
Rats, Wistar
Retina / pathology
Transforming Growth Factor beta / biosynthesis*
Vitreoretinopathy, Proliferative / chemically induced,  pathology*
Vitreous Body / pathology,  physiology
Reg. No./Substance:
0/Glial Fibrillary Acidic Protein; 0/Platelet-Derived Growth Factor; 0/Transforming Growth Factor beta; 0/platelet-derived growth factor A; 0/platelet-derived growth factor BB

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