| Immunohistochemical analysis of rat and human respiratory cilia with anti-dynein antibody: comparison between normal cilia and pathological cilia in primary ciliary dyskinesia. | |
| | |
MedLine Citation:
|
PMID: 8548125 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Wistar Imamichi rat and human respiratory cilia were examined with anti-dynein antibody (AD2), which is specific for sea urchin sperm flagellar dynein. AD2-labelled fresh-frozen normal rat and human cilia stained clearly by immunofluorescence and the peroxidase-antiperoxidase (PAP) technique. On immunoelectron microscopy, AD2 labelled the outer dynein arms of normal human cilia. Paraffin-embedded normal human cilia also stained by immunofluorescence, although not always clearly. Neither the cilia of WIC-Hyd male rats, an animal model of Kartagener's syndrome, nor human cilia from patients with primary ciliary dyskinesia (PCD) reacted positively by the immunofluorescence or PAP technique. Western blots of normal rat cilia yielded a single band of about 450 kDa. In conclusion, AD2 recognizes the outer arm dynein heavy chains of healthy cilia and may be useful in diagnosing and classifying PCD light microscopically especially when only paraffin-embedded specimens are available. This approach may be of potential use for better defining and classifying PCD. |
| | |
Authors:
|
A Umeda; T Yoshida; K Yamaguchi; M Kanazawa; C Torikata |
Related Documents
:
|
3923485 - Purification, induction, and distribution of placental glutathione transferase: a new m... 2333015 - Phosphatidylcholine synthesis in the rat: the substrate for methylation and regulation ... 76675 - Relationship between functional castration and alpha-fetoprotein produced by hepatoma-b... 613335 - Nmr in cancer: viii. phosphorus-31 as a nuclear probe for malignant tumors. 11033005 - Does fucose or piracetam modify the effect of hypoxia preconditioning against pentylene... 2928625 - Sleep deprivation in the rat: ii. methodology. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Virchows Archiv : an international journal of pathology Volume: 427 ISSN: 0945-6317 ISO Abbreviation: Virchows Arch. Publication Date: 1995 |
Date Detail:
|
Created Date: 1996-02-21 Completed Date: 1996-02-21 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 9423843 Medline TA: Virchows Arch Country: GERMANY |
Other Details:
|
Languages: eng Pagination: 401-6 Citation Subset: IM |
Affiliation:
|
Department of Medicine, School of Medicine, Keio University, Tokyo, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adolescent Adult Aged Animals Cilia / chemistry Ciliary Motility Disorders* Dyneins / analysis* Humans Immunohistochemistry Kartagener Syndrome Lung / chemistry* Male Microscopy, Electron Middle Aged Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
|
EC 3.6.4.2/Dyneins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Basic fibroblast growth factor-heparan sulphate complex in the human dialysis-related amyloidosis.
Next Document: Improved detection of medically important fungi by immunoperoxidase staining with polyclonal antibod...